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SRP6433 Sigma-Aldrich

PD-L1/CD274/B7-H1 from mouse

recombinant, expressed in HEK 293 cells, ≥95% (SDS-PAGE)

Synonym: PDCD1L1, PDCD1LG1

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Properties

Related Categories CD160 to CD340, Cell Biology, Cell Signaling and Neuroscience, Cluster of Differentiation (CD) Proteins, Cytokines and Growth Factors,
biological source   mouse
recombinant   expressed in HEK 293 cells
assay   ≥95% (SDS-PAGE)
form   lyophilized
mol wt   calculated mol wt 25.6 kDa
  observed mol wt 40-50 kDa (DTT-reduced. Protein migrates due to glycosylation. Phe 19 is the predicted N-terminal.)
packaging   pkg of 10 and 50 μg
impurities   <1 EU/μg (LAL test)
conjugate   6-His tagged (C-terminus)
UniProt accession no.   Q9EP73
shipped in   wet ice
storage temp.   −20°C
Gene Information   mouse ... CD274(60533)

Description

General description

Programmed cell death 1 ligand 1 (PD-L1), which is also known as cluster of differentiation (CD274) or B7 homolog 1 (B7-H1), is a member of the growing B7 family of immune molecules. PD-L1 is a cell surface immunoglobulin with two Ig-like domains within the extracellular region and a short cytoplasmic domain. The protein is highly expressed in the heart, skeletal muscle, placenta and lung and weakly expressed in the thymus, spleen, kidney and liver. PD-L1 is expressed on activated T-cells, B-cells, dendritic cells, keratinocytes and monocytes.

Biochem/physiol Actions

Programmed cell death 1 ligand 1 (PD-L1) is involved in the regulation of cellular and humoral immune responses. PD-L1 is up-regulated on T- and B-cells, dendritic cells, keratinocytes and monocytes after lipopolysaccharide (LPS) and interferon-γ (IFNG) activation and up-regulated in B-cells activated by surface Ig cross-linking. PD-L1 is involved in the co-stimulatory signal, essential for T-cell proliferation and production of interleukin-10 (IL10) and IFNG, in an interleukin-2-dependent and a programmed cell death 1 (PDCD1)-independent manner.

Physical form

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Mannitol or Trehalose is added as protectants before lyophilization.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 μg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis (COA)

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Protocols & Articles
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