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SRP7783 Sigma-Aldrich

MMP-3 human

recombinant, expressed in E. coli, ≥95% (SDS-PAGE), ≥95% (HPLC)

Synonym: EC 3, Matrix metalloproteinase-3, Stromelysin-1

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Properties

Related Categories Application Index, Biochemicals and Reagents, Cell Biology, Cell Signaling Enzymes, Cell Signaling and Neuroscience,
biological source   human
recombinant   expressed in E. coli
assay   ≥95% (HPLC)
  ≥95% (SDS-PAGE)
form   lyophilized
mol wt   ~19.5 kDa
packaging   pkg of 10 μg
application(s)   western blot (chemiluminescent): suitable
impurities   endotoxin, tested
NCBI accession no.   P08254
shipped in   wet ice
storage temp.   −20°C
Gene Information   human ... MMP3(4314)

Description

General description

Active MMP-3 catalytic domain from human cDNA, expressed in E. coli and purified using proprietary technologies. The active MMP-3 is very useful in studying enzyme kinetics, cleave target substrates, and screen for inhibitors.

The gene JE is an early growth response gene that was first identified in mouse 3T3 cells. The gene is mapped to mouse chromosome 11. It encodes a member of the CC subfamily, the members of which contain characteristic two adjacent cysteine residues.

Application

MCP-1 has been used to induce chemotaxis in mice macrophages.

MMP-3 human has been used to develop a simple and sensitive electrogenerated chemiluminescence (electrochemiluminescence (ECL)) peptide-based bioassay for the detection of matrix metalloproteinases releasing from living cells.

Biochem/physiol Actions

Matrix metallopeptidase 3 (MMP3) has a broad substrate specificity and is capable of degrading fibronectin, laminin, collagens III, IV, IX, and X, and cartilage proteoglycans. It participates in wound repair, progression of atherosclerosis, tissue remodeling and tumor initiation. MMP3 variants can be associated with hypertrophy of interventricular septum or hypertrophic cardiomyopathy.

The gene JE, also referred to as MCP-1 (monocyte chemoattractant protein-1), encodes a secreted chemokine that functions as a chemoattractant for monocytes and may activate their anti-tumor properties. This protein may serve as a potential therapeutic anticancer agent.

Physical form

Lyophilized powder.

Reconstitution

Centrifuge the vial prior to opening. Avoid freeze-thaw cycles.

Reconstitute in sterile PBS to a concentration of 0.1-1.0 mg/mL. This solution can then be diluted into other aqueous buffers.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Protocols & Articles
Peer-Reviewed Papers
15

References

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