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T0157 Sigma-Aldrich

Tumor Necrosis Factor-α human

TNF-α, recombinant, expressed in yeast, buffered aqueous solution, suitable for cell culture

Synonym: TNF-α, hTNF-α

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Properties

Related Categories Cancer Research, Cell Biology, Cell Culture, Cell Signaling and Neuroscience, Cytokines & Growth Factors,
Quality Level   200
biological source   human
recombinant   expressed in yeast
assay   ≥95% (SDS-PAGE)
form   buffered aqueous solution
potency   >1x108 units/mg ED50
quality   endotoxin tested
specific activity   ≥2 × 107 U/mg
mol wt   ~17.4 kDa
packaging   pkg of 10 μg
storage condition   avoid repeated freeze/thaw cycles
concentration   0.1-10 mg/mL
application(s)   cell culture | mammalian: suitable
impurities   <10 EU/mLtested (LAL test)
color   colorless
UniProt accession no.   P01375
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... TNF(7124)

Description

General description

TNFα (tumor necrosis factor α) belongs to the TNF superfamily and is mapped to human chromosome 6p21.33.

Application

Tumor Necrosis Factor-α human has been used to study the differential regulation of microRNA-146a , CFH (complement factor-H), IRAK-1 (interleukin-1 receptor associated kinase-1) and tetraspanin-12 in primary brain cell cultures in presence of TNF-α. To determine the intercellular adhesion molecule-1 (ICAM1) expression using ELISA.

Biochem/physiol Actions

TNF-α is associated with formation, growth and destabilization of atherosclerotic plaques. TNF-α is involved in the disease progression of osteoarthritis.

Tumore necrosis factor-α (TNF-α), also known as cachectin, is expressed as a 26 kDa membrane bound protein and is then cleaved by TNF-α converting enzyme (TACE) to release the soluble 17 kDa monomer, which forms homotrimers in circulation. TNF-α plays roles in antitumor activity, immune modulation, inflammation, anorexia, cachexia, septic shock, viral replication and hematopoiesis. TNF-α is expressed by a great variety of cells, with numerous inductive and suppressive agents. Primarily, TNF-α is produced by macrophages in response to immunological challenges such as bacteria (lipopolysaccharides), viruses, parasites, mitogens and other cytokines. TNF-α is cytotoxic for many transformed cells (its namesake activity) but in normal diploid cells, it can stimulate proliferation (fibroblasts), differentiation (myeloid cells) or activation (neutrophils). TNF-α also shows antiviral effects against both DNA and RNA viruses and it induces production of several other cytokines. Although TNF-α is used in clinical trials as an antitumor agent, Sigma′s cytokine, growth factor and hormone products are for research only. TNF-α and the related molecule TNF-β (LT-α) share close structural homology with 28% amino acid sequence identity and both activate the same TNF receptors, TNFR1 and TNFR2. Mouse and human TNF-α share 79% amino acid sequence identity. Unlike human TNF-α, the mouse form is N-glycosylated.

Physical form

Solution in phosphate buffered saline containing 1 mg/ml bovine serum albumin.

Analysis Note

The cytolytic activity of TNF-α against WEHI 164 cells has been measured in culture using an MTT toxicity assay. The ED50 is defined as the concentration of TNF-alpha that mediates half-maximal cytotoxicity in the presence of 1 micro-g/ml actinomycin D.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
RTECS 
MT6435000
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles

Articles

Tumor Necrosis Factor (TNF) Superfamily

The tumor necrosis factor (TNF) superfamily comprise an increasing number of structurally related ligand-receptor pairs, including TNF-α, TNF-β (lymphotoxin, LT), TNFR1, TNFR2, LT-βR, osteoprotegrin ...
Jennifer Fries
BioFiles 2009, 4.5, 23.
Keywords: Apoptosis, Biofiles, Cell disruption, Ligands, Microscopy, Type

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Peer-Reviewed Papers
15

References

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