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  • T0167 - D-(+)-Trehalose dihydrate

T0167 Sigma-Aldrich

D-(+)-Trehalose dihydrate

from Saccharomyces cerevisiae, powder, BioReagent, suitable for cell culture, suitable for insect cell culture, ≥99%

Synonym: α,α-Trehalose, α-D-Glucopyranosyl-α-D-glucopyranoside

  • CAS Number 6138-23-4

  • Empirical Formula (Hill Notation) C12H22O11 · 2H2O

  • Molecular Weight 378.33

  •  Beilstein/REAXYS Number 5322018

  •  EC Number 202-739-6

  •  MDL number MFCD00071594

  •  PubChem Substance ID 24899871

  •  NACRES NA.75



Related Categories Cell Culture, Companion Products and Reagents, Insect Platform, Miscellaneous Reagents and Supplements, Reagents and Supplements,
Quality Level   200
biological source   Saccharomyces cerevisiae
  Saccharomyces cerevisiae
product line   BioReagent
assay   ≥99%
form   powder
application(s)   cell culture | insect: suitable
  cell culture | mammalian: suitable
solubility   H2O: 50 mg/mL
SMILES string   [H]O[H].[H]O[H].OC[C@H]1O[C@H](O[C@H]2O[C@H](CO)[C@@H](O)[C@H](O)[C@H]2O)[C@H](O)[C@@H](O)[C@@H]1O
InChI   1S/C12H22O11.2H2O/c13-1-3-5(15)7(17)9(19)11(21-3)23-12-10(20)8(18)6(16)4(2-14)22-12;;/h3-20H,1-2H2;2*1H2/t3-,4-,5-,6-,7+,8+,9-,10-,11-,12-;;/m1../s1


General description

Trehalose is a non-reducing disaccharide, ubiquitously present in nature. It is used as a food preservative and also to retain enzymes, vaccines, cells and many other molecules. Trehalose is present as hemolymph sugar among the invertebrates.


D-(+)-Trehalose dihydrate has been used in a slow-freezing method for freezing rabbit oocytes. It has also been used in solid-surface vitrification (SSV) of in vitro-matured oocytes.


10, 25, 100 g in poly bottle

Biochem/physiol Actions

Trehalose is essential for embryogenesis, carbon metabolism, photosynthesis and flowering in plants. It predominantly exists among the species that supports anhydrobiosis (life without water). It serves as a carbohydrate reserve in microorganisms and protects them from adverse conditions, such as heat, cold, dehydration, desiccation and oxidation. Trehalose acts as a chemical chaperone affecting protein folding. It is known to prevent amyloid generation and accummulation of β-amyloid in vitro. A similar effect of trehalose has been observed with huntingtin protein aggregation. Thus, it might serve as a protective agent in Alzheimer′s disease and Huntington′s disease.

Safety & Documentation

Safety Information

Personal Protective Equipment 
NONH for all modes of transport
WGK Germany 
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable


Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
How do I find price and availability?
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What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
Can solutions of Product T0167, D-(+)-Trehalose dihydrate, be autoclaved?
Do not autoclave. It is best to sterile filter solutions.
Do we have information of any fluorescent impurities in Product T0167, D-(+)-Trehalose dihydrate?
This product is not tested for the presence or absence of fluorescent impurities.
CanProduct T0167, D-(+)-Trehalose dihydrate, be used as a substitute for protein in a freezing media?
Yes, it should work as a substitute for protein in a freezing media.It is commonly used at a final concentration of 5% (50 mg/mL).Alternatively, one could use methylcellulose.
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