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  • T0198 - Monoclonal Anti-β-Tubulin antibody produced in mouse

T0198 Sigma-Aldrich

Monoclonal Anti-β-Tubulin antibody produced in mouse

clone D66, purified from hybridoma cell culture



Related Categories Alphabetical Index, Antibodies, Antibodies against Proteins/Bioactives/Markers/Receptors for Stem Cell Biology, Antibodies for Cell Biology, Antibodies to Cell and Organelle Proteins,
conjugate   unconjugated
clone   D66, monoclonal
biological source   mouse
application(s)   immunocytochemistry: suitable (3% paraformaldehyde-0.5% Triton X-100 and methanol-acetone)
  immunoprecipitation (IP): suitable
  indirect ELISA: suitable
  western blot: 0.5-1.0 μg/mL using whole extract of cultured rat adrenal pheochromocytoma (PC-12) cells
species reactivity   monkey, Oxyrrhis marina (dinoflagellate), rat, mouse, chicken, sea urchin (Lytechinus pictus and Paracentrotus lividus), rabbit, canine, Chlamydomonas reinhardtii, human, bovine, hamster
mol wt   antigen mol wt ~50 kDa
form   buffered aqueous solution
shipped in   dry ice
storage temp.   −20°C
antibody form   purified immunoglobulin
isotype   IgG1
antibody product type   primary antibodies
concentration   ~1 mg/mL
UniProt accession no.   Q9H4B7
Gene Information   human ... TUBB1(81027), TUBB2A(7280), TUBB2C(10383)
mouse ... Tubb1(545486), Tubb2a(22151), Tubb2c(227613)
rat ... Tubb2(29212), Tubb2c(296554)


General description

β-Tubulin belongs to the subfamily of tubulins, which is the major building block of microtubules. β-tubulin has a molecular weight of 55kDa. β−Tubulin structure is characterized with core of two β -sheets enclosed by a-helices. It also contains an N-terminal domain with the guanine nucleotide-binding region, an intermediate domain with taxol-binding site, and a C-terminal domain that contains the binding surface for molecular motor proteins. Human β-tubulin consists of seven isoforms (β I (class I), β II (class II), β III (class III), β IVa (class IVa), β IVb (class IVb), β V (class V), and β VI (class VI)).


Recognizes all mouse brain β-tubulin isoforms (β and β′ subpopulations), and sea urchin β2-tubulin. The epitope recognized by the antibody is localized in the C-terminal region of β-tubulin (amino acids 427-432 in sea urchin β2-tubulin).


sea urchin (Lytechinus pictus) sperm axonemal proteins.


Monoclonal Anti-β-tubulin antibody produced in mouse has been used in western blotting and immunocytochemistry.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% BSA and 15 mM sodium azide.


Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

Mutation in the gene leads to various neuronal migration disorders such as lissencephaly, pachygyria and polymicrogyria malformations. B2702 peptide binds to β- tubulin and inhibits natural killer (NK) cell cytotoxicity and it influences microtubule polymerization, which damages cytoskeleton organization and chaperone-like activity of tubulin. β-1 tubulin, also known as class VI or TUBB1, plays a vital role in platelet production, and is considered to be a potential biomarker candidate for immune thrombocytopenia (ITP).

Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 

Milli-Q® Water Purification Solutions
Protocols & Articles


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