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  • T0438 - Monoclonal Anti-Transforming Growth Factor-β1 antibody produced in mouse

T0438 Sigma

Monoclonal Anti-Transforming Growth Factor-β1 antibody produced in mouse

clone 9016, purified immunoglobulin, lyophilized powder

Synonym: Anti-TGF-β1, CEDLAP, DPD1, latency-associated peptide



Related Categories Alphabetical Index, Antibodies, Antibodies for Apoptosis, Antibodies for Cell Biology, Antibodies for Hematopoietic Stem Cells,
species reactivity   rat, mouse, human
application(s)   ELISA: suitable
  flow cytometry: suitable
  immunocytochemistry: suitable
  immunohistochemistry: suitable
  neutralization: suitable
  western blot: suitable
clone   9016, monoclonal
antibody form   purified immunoglobulin
form   lyophilized powder
isotype   IgG1
storage temp.   −20°C
Gene Information   human ... TGFB1(7040)
mouse ... Tgfb1(21803)
rat ... Tgfb1(59086)
biological source   mouse
conjugate   unconjugated
Featured Industry   Research Pathology



Chinese hamster ovary cell line CHO-derived recombinant TGF- beta 1 and latent TGF- beta 1

General description

Transforming growth factor-β (TGF-β) belongs to the TGF-β superfamily of secreted proteins. TGF-β 1 exists as three isoforms TGF-β 1, TGF-β 2 and TGF-β 3. The TGF-β family is involved in a variety of signaling pathways that involve serine/threonine protein kinases. TGF-β acts through binding to three transmembrane receptors in mammals, type I, type II and type III receptors. The important pathways regulated by TGF-β are the SMAD, BMP, MAPK and PI3K/Akt pathways. TGF-β 1 is involved in cell and tissue differentiation, proliferation, inflammation, migration of monocytes, neutrophils and chemotaxis. Deregulation of TGF-β signaling has been implicated in many inflammation and autoimmune diseases. Monoclonal Anti-Transforming Growth Factor-β 1 antibody specifically reacts with human, mouse and rat TGF-β 1. The antibody shows less than 2% cross reactivity with TGF-β3 and TGF-β5 in ELISA.

Physical form

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing carbohydrates.


By capture ELISA, <2% cross-reactivity is observed with TGF-β3 and TGF-β5.


Recommended concentrations:
Western Blot 1 ug/mL (under non-reducing conditions only)
Immunohistochemistry 3-25 μg/mL
Flow Cytometry 2.5 μg/106 cells
ELISA capture 2-8 μg/mL
ELISA detection 0.1-0.4 μg/mL
Neutrilization 0.3-1.0 μg/mL

Other Notes

Protein A or G purified from hybridoma culture supernatant.


Reconstitute at 0.5 mg/mL in sterile PBS.

Price and Availability

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Safety & Documentation

Safety Information

Personal Protective Equipment  Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter
RIDADR  NONH for all modes of transport
WGK Germany  3
Protocols & Articles


Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy


Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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