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T0699 Sigma-Aldrich

Trichloroacetic acid solution

6.1 N

Synonym: TCA

  • CAS Number 76-03-9

  • Linear Formula Cl3CCOOH

  • Molecular Weight 163.39

  •  Beilstein Registry Number 970119

  •  MDL number MFCD00004177

  •  PubChem Substance ID 57654645




Traditionally used to precipitate protein. Has been used to determine protein concentration by quantitative precipitation.
Used as a decalcifier and fixative in microscopy.


100 mL in glass bottle

Safety & Documentation

Safety Information

Signal word 
Hazard statements 
UN 2564 8 / PGII
WGK Germany 
Protocols & Articles


Enzymatic Activity of Glucose-6-Phosphatase [EC]

Replaces enzyme assay procedure SSGLUC02. Document number and formatting changed to reflect current numbering and formatting guidelines. The procedure was modified to include a standard curve that re...
Keywords: Enzyme activity, Enzymology

Enzymatic Assay of Amyloglucosidase (EC

This procedure may be used for the determination of Amyloglucosidase activity using starch as the substrate. The Spectrophotometric Stop Rate Determination [Absorbance at 340 nm (A340), Light path = ...
Keywords: Enzyme activity, Extinction coefficient

Enzymatic Assay of Choloylglycine Hydrolase

To standardize a procedure for the determination of the enzymatic assay of choloylglycine hydrolase.
Keywords: Enzyme activity

Enzymatic Assay of Ficin (EC

The spectrophotometric stop rate detemination (A280, Light path = 1 cm) is based on the following reaction:

Enzymatic Assay of Pepsin (

This procedure may be used for determination of Pepsin activity using hemoglobin as the substrate. It is a spectrophotometric stop rate determination.

Enzymatic Assay of Protease Using Casein As a Substrate

To standardize a procedure for the enzymatic assay of Protease using Casein as a substrate at Sigma-Aldrich St. Louis.

Enzymatic Assay of Proteinase K (EC

This procedure may be used for determination of Proteinase K activity using hemoglobin as the substrate. It is not used to assay the activity of immobilized Proteinase K products, such as Catalog Num...
Keywords: Immobilization

Enzymatic Assay of Proteinase K with Hemoglobin Substrate

The objectiveis to standardize a procedure for the enzymatic determination of Proteinase K using Hemoglobin as the substrate.
Keywords: Filtration

Peer-Reviewed Papers


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