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  • T0813 - Anti-Tumor Necrosis Factor-α antibody produced in goat

T0813 Sigma-Aldrich

Anti-Tumor Necrosis Factor-α antibody produced in goat

affinity isolated antibody, lyophilized powder

Synonym: Anti-TNF-α

  •  NACRES NA.41



Related Categories Alphabetical Index, Antibodies, Antibodies for Apoptosis, Antibodies for Cell Biology, Antibodies to Cytokines and Growth Factors,
conjugate   unconjugated
clone   polyclonal
biological source   goat
application(s)   immunohistochemistry: suitable
  neutralization: suitable
  western blot: suitable
species reactivity   human
form   lyophilized powder
storage temp.   −20°C
antibody form   affinity isolated antibody
Quality Level   100
antibody product type   primary antibodies
UniProt accession no.   P01375
Gene Information   human ... TNF(7124)


General description

Tumor Necrosis Factor-α (TNF-α) is a cytokine produced by macrophages in response to stimulus by LPS. It plays a major role in mediating inflammation, tissue injury, pathogenic shock, innate immunity, apoptosis and autoimmunity. The physiological effects of TNF-α are mediated by receptors of TNFR super family. The TNF/TNFR signaling axis plays a critical role in mediating either survival or apoptosis depending on the class of adaptor proteins recruited in the cell. Association of receptors with a death domain causes rapid activation of caspases and results in cell death. TNF/TNFR also results in the activation of downstream pathways involving MAPK, p38, JNK and NF-κB that play a major role in innate immunity, acute inflammatory responses and homeostasis. The TNF/TNFR axis is also important in the pathology of various autoimmune and inflammatory disorders including a variety of malignancies and Alzheimer′s disease. Anti-Tumor Necrosis Factor-α antibody is specific for human TNF-α. The antibody shows less than 10% cross reactivity with recombinant rabbit TNF-α and less than 5% cross reactivity with recombinant mouse TNF-α.


The antibody neutralizes the biological activity of recombinant human TNF-α. The antibody shows <10% cross-reactivity with recombinant rat TNF-α and <5% cross-reactivity with recombinant mouse TNF-α.


recombinant human TNF-α.


Anti-Tumor Necrosis Factor-α antibody may be used for immunoblotting at a working antibody concentration of 0.1 μg/ml. For immunohistochemistry a concentration range of 5-15 μg/ml is recommended. The antibody may be used for neutralization assay; the ND50 is 0.01-0.06 μg/mL in the presence of 1.5ng/mL Recombinant Human TNF-alpha.

Anti-Tumor Necrosis Factor-α (TNF- α) antibody produced in goat has been used to study the release of TNF- α from the cocultured lung carcinoma cells A427:AML-193.

Physical form

Lyophilized from 0.2 μm filtered solution in phosphate buffered saline containing 5% trehalose.


Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Preparation Note

The antibody is purified using TNF-α affinity chromatography.

Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable


Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles


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