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  • T0951 - Anti-Tet Repressor (TETR) antibody produced in rabbit

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T0951 Sigma-Aldrich

Anti-Tet Repressor (TETR) antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

Synonym: Anti-Tetracycline Repressor Protein

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies against Proteins/Bioactives/Markers/Receptors for Stem Cell Biology, Antibodies for Cell Biology, Antibodies for Epigenetics and Nuclear Signaling,
conjugate   unconjugated
clone   polyclonal
biological source   rabbit
application(s)   indirect ELISA: suitable
  western blot: 1:1,000
species reactivity   human
mol wt   antigen ~22 kDa by calculation
form   buffered aqueous solution
shipped in   dry ice
storage temp.   −20°C
antibody form   affinity isolated antibody
antibody product type   primary antibodies

Description

General description

The Tet repressor protein (TetR) regulates transcription of a family of tetracycline resistance determinants in Gram-negative bacteria The Tet system is the most widely used regulatory system for conditional gene expression in eukaryotic cells. TetR can be used for that purpose in some organisms without further modifications In mammals and in a large variety of other organisms, however, eukaryotic transcriptional activator or repressor domains are fused to TetR to turn it into an efficient regulator

Immunogen

Anti-Tet Repressor (TETR) reacts with amino acid residues 31-49 (AQKLGVEQPTLYWHVKNKR) of 22 kDa Tet repressor protein

Physical form

Solution of 1 mg/mL in phosphate buffered saline containing 0.02% sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
nwg

Documents

Certificate of Analysis

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Certificate of Origin

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Milli-Q® Water Purification Solutions
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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