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T0956 Sigma

Sigma Universal Transfection Reagent

Polymer reagent for transfecting common mammalian cell lines

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Properties

Related Categories Cell Biology, Cloning and Expression, Life Science Research, Molecular Biology, Synthetic Biology,
grade   for molecular biology
sterility   aseptically filled
form   liquid (buffered aqueous solution)
usage   sufficient for 110 transfections (3.5 cm dish)
  sufficient for 40 transfections (10 cm dish)
  sufficient for 65 transfections (6 cm dish)
concentration   2 mg/mL
storage temp.   −20°C

Description

Components

Note the formulation of Sigma Universal Transfection Reagent is confidential.

Application

Suitable for transient and stable transfection of nucleic acids into cultured eukaryotic cells. Use approximately 1-3 μl Universal Transfection Reagent per μg DNA. Protocol optimization provides very efficient transfection. The following cells have been successfully transfected using Sigma Universal Transfection Reagent:

Excellent for:
BHK-21
CHO
CHOK1
HEK293
HeLa
NIH3T3

Good for:
3LL
6CSFME
ATT20B16-F0
B16-F10
BAEC
Ca Ski
COS-7
CV-1
D 407
HUVEC
L929
MCF-7
MDCK
Neuro2A
SAOS-2
SHEP
SiHa
SKOV3

General description

Sigma Universal Transfection Reagent is a unique formulation of a biodegradable, proprietary cationic polymer conjugated to a cationic lipid. Universal Transfection Reagent is used for transfection of nucleic acids into common eukaryotic cell lines. Fast and easy protocol is compatible with serum, serum-free medium and antibiotics. For most cells, medium change after transfection is not necessary.

Features and Benefits

• Fast, easy protocol
• Suitable for stable and transient transfection
• High efficiency for a wide variety of cell types including primary cells
• Compatible with both serum and serum-free transfection protocols

Principle

Sigma Universal Transfection Reagent is a cationic polymer conjugated to a cationic lipid. When mixed with plasmid DNA, the reagent forms stable complexes that can be directly added to the cell culture. The cells take up the complexes by endocytosis, the polymer is cleaved from the complex, and the plasmid DNA is quickly released into the cytoplasm.

Other Notes

Complex formation is inhibited by serum, but once stable complexes have formed, the presence of serum lowers the toxicity of transfection.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Certificate of Origin


Milli-Q® Water Purification Solutions
Protocols & Articles

Articles

Introduction to Transfection - Transfection Reagents

Transfection involves the introduction of DNA or RNA into eukaryotic cells and is used in research to study and modulate gene expression. Thus, transfection techniques serve as an analytical tool tha...
Keywords: Apoptosis, Biological techniques, Drug discovery, Gene expression, Genetic, Precipitation, Transfection, transformation

Transfection Best Practices

The work of scientific research is an art which requires good attention to detail.  The quality of transfection is highest when these commonly used best practices and notes on optimization are employ...
Keywords: Antibiotics, Apoptosis, Growth factors, Molecular biology, Purification, Transfection

Transfection Reagent Selection Guide

Choosing a transfection reagent is easier when you can rely on the work of researchers who have used it before you. During development of each reagent, good transfection efficiency was verified in a ...
Keywords: Transfection

Protocols

Selecting Correctly Expressing Recombinants

Blue / White colony screening is a strategy to quickly and easily distinguish between recombinant and non-recombinant colonies. It requires a special vector and a special strain of E. coli. It is par...
Keywords: AGE, Amplification, Antibiotics, Cell disruption, Centrifugation, Cloning, Degradations, Detergents, Diagnostic, Digestions, Electrophoresis, Gel electrophoresis, Molecular biology, Peptide synthesis, Phase transitions, Polymerase chain reaction, Precipitation, Purification, Sequencing, Transfection, transformation

Universal Transfection Reagent Protocol

Sigma's Universal Transfection Reagent is a unique formulation of a proprietary polymer blend. Universal Transfection Reagent is used for transient and stable transfection of nucleic acids into vario...
Keywords: Antibiotics, Cell culture, Gene expression, Molecular biology, Transfection

Universal Transfection Reagent | Lentivirus Generation in 293T Cells Protocol

Sigma's Universal Transfection Reagent is a unique formulation of a proprietary polymer blend. Universal Transfection Reagent is used for transient and stable transfection of nucleic acids into vario...
Keywords: Antibiotics, Applications, Cell culture, Gene expression, Molecular biology, Transfection, Type

Universal Transfection Reagent | Mammalian Hard-to-Transfect Cells Protocol

Sigma's Universal Transfection Reagent is a unique formulation of a proprietary polymer blend. Universal Transfection Reagent is used for transient and stable transfection of nucleic acids into vario...
Keywords: Antibiotics, Applications, Cell culture, Gene expression, Molecular biology, Transfection, Type

Related Content

Synthetic Biology Resources

Sigma® Life Science is working to use our stregnths to build the field of Synthetic Biology. Below is a collection of links to our favorite resources. Sigma also offers thousands of products for your...
Keywords: Cell culture, Cloning, Gene expression, Genetic, Genetics, Metabolic Pathways, Metabolites, Molecular biology, Molecular biology techniques, Photosynthesis, Poisons, Polymerase chain reaction - quantitative, Transcription, Transfection

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