T1500 Sigma



Synonym: 17β-Hydroxy-3-oxo-4-androstene, 17β-Hydroxy-4-androsten-3-one, 4-Androsten-17β-ol-3-one, trans-Testosterone



Related Categories Androgen, Androgen Modulators, Application Index, Bioactive Small Molecule Alphabetical Index, Bioactive Small Molecules,
assay   ≥98%
form   powder
drug control   USDEA Schedule III; Home Office Schedule 4.2; regulated under CDSA - not available from Sigma-Aldrich Canada
solubility   45% (w/v) aq 2-hydroxypropyl-β-cyclodextrin: soluble 18.2 mg/ml
  absolute ethanol: soluble 0.5 mg/ml
  chloroform: soluble 100 mg/mL, clear, colorless to very faintly yellow
  H2O: insoluble
  dioxane: soluble
  methanol: soluble
  methyl oleate: slightly soluble
  vegetable oils: soluble
Gene Information   human ... AR(367), EBP(10682), ESR1(2099), PGR(5241), SERPINA6(866), SHBG(6462)
mouse ... Esr1(13982)
rat ... Ar(24208), Esr1(24890), Nr3c1(24413), Nr3c2(25672), Pgr(25154)


Biochem/physiol Actions

Testosterone secreted by the testis is converted to dihydrotestosterone in the target tissues where it appears to mediate many of the biological actions of testosterone. Androgens direct the development of the male phenotype during embryogenesis and at puberty.

Preparation Note

Testosterone dissolves in chloroform at 100 mg/ml to yield a clear, colorless to very faint yellow solution. It is also soluble in methanol, absolute ethanol (0.5 mg/ml), 45 percent (w/v) aqueous 2-hydroxypropyl-b-cyclodextrin (18.2 mg/ml), vegetable oils and dioxane. It is slightly soluble in methyl oleate and insoluble in water.


Testosterone has been used as a standard to study the developmental profile of steroid hormones in male and female rat brains. This hormone has also been used to evaluate its effects on muscle mass and signaling in mice.

Features and Benefits

This compound is a featured product for ADME Tox research. Click here to discover more featured ADME Tox products. Learn more about bioactive small molecules for other areas of research at

This compound is featured on the Nuclear Receptors (Steroids) page of the Handbook of Receptor Classification and Signal Transduction. To browse other handbook pages, click here.

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Safety Information

Signal word 
Hazard statements 
Precautionary statements 
UN 3077 9 / PGIII
WGK Germany 


Certificate of Analysis

Certificate of Origin

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis. These documents are located on the product detail page under Useful Links & Tools. Click on the following link to search for a Certificate of Analysis. Please click the following link to see the details on our Product Dating Information.
How do I get lot-specific information or a Certificate of Analysis?
A Certificate of Analysis is available by lot number and can be obtained through our Advanced Search Option:
How do I find price and availability?
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What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS. To access the shipping information for this material, use the link on the product detail page for the product, or search here. 
My question is not addressed here, how can I contact Technical Service for assistance?
Use the option to the right to "Ask a Question" by email of a Technical Service Scientist.
What is the method of production for Product T1500, Testosterone?
This information is listed on the product's Certificate of Origin, which is lot-specific and, thus, subject to change. Enter the lot number of interest into the Certificate of Origin search box on the product page to find this information.
How long can I store a solution of Product T1500, Testosterone?
We have not determined the solution stability of this product. As a guideline, please note the stability of product no. T5411, Testosterone solution 1.0 mg/mL, which is stable for  3 years at 2-8 C.
What can I use to prepare a solution of Product T1500, Testosterone?
The product is soluble in absolute ethanol (0.5 mg/ml), It is also soluble in vegetable oils, soluble in dioxan and slightly soluble in methyl oleate.
It is possible to sterile filter a solution of Product T1500, Testosterone?
A solution of testosterone cannot be sterile filtered, since the compound will stick to the filter paper, according to Sigma cell culture scientists' experience.
How could I dissolve Product T1500, Testosterone for cell culture use?
This product has not been tested for cell culture use. We offered Testosterone for cell culture, now discontinued, and for it our 1998 Sigma Cell Culture advised to dissolve 1 mg in 2.0 mL absolute ethanol, gently swirl to dissolve, then add 48 mL sterile medium to prepare 20 ug/ml stock solution. Working range: 20-300 ng/ml.
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730610 Testosterone-2,3,4-13C3 solution, 0.1 mg/mL in methanol, 99 atom % 13C, 98% (CP)

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