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T1568 Sigma-Aldrich

Thrombopoietin human

recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture, ≥98% (SDS-PAGE and HPLC)

Synonym: MGDF, Megakaryocyte colony-stimulating factor, TPO, c-MPL ligand

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Properties

Related Categories Bioactives and Supplements for Stem Cell Expansion, Bioactives/Supplements, Cell Biology, Cell Culture, Cell Signaling and Neuroscience,
biological source   human
recombinant   expressed in E. coli
assay   ≥98% (SDS-PAGE and HPLC)
form   lyophilized powder
potency   ≤1.0 ng/mL ED50
mol wt   18.6 kDa
packaging   pkg of 5 μg
storage condition   avoid repeated freeze/thaw cycles
impurities   endotoxin, tested
suitability   suitable for cell culture
UniProt accession no.   P40225
storage temp.   −20°C
Gene Information   human ... THPO(7066)

Description

Application

TPO stimulates the proliferation and maturation of megakaryocytes and promotes increased circulating levels of platelets in vivo. TPO signals through the c-mpl receptor and acts as an important regulator of circulating platelets

Biochem/physiol Actions

Thrombopoeitin is a primary regulatory factor for megakaryocytopoiesis and thrombopoiesis. The mature form of TPO is a highly conserved glycoprotein, showing homology among various mammals. It is produced by liver and kidney cells. TPO stimulates growth and maturation of megakaryocytes and megakaryocytic colonies from bone marrow cultures. TPO binds and activates an 68-78 kDa glycoprotein receptor belonging to the GH family of cytokine receptors, a family that includes receptors to growth hormone (GH), erythropoietin (EPO), and prolactin (PRL). Like GH and EPO, TPO may bind to its receptor at two distinct sites, initiating receptor dimerization and activation. Analysis of mRNA indicates also the existence of a novel truncated and potentially soluble form of TPO receptor. The viral oncogene v-mlp of the myeloproliferative leukemia virus (MPLV) contains the gene sequence for the entire cytoplasmic and transmembrane domains and a portion of the extracellular domain of c-mlp (TPO receptor).

Physical form

Lyophilized from a 0.1 % TFA solution containing 250 μg bovine serum albumin

Analysis Note

The biological activity is measured in a cell proliferation assay using MO7e cells.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

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Certificate of Origin

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Cell Culture Guide
Protocols & Articles

Articles

Hematopoietic Cytokines

Hematopoietic cytokines are large family of extracellular ligands that stimulate hematopoietic cells to differentiate into eight principle types of blood cells. Numerous cytokines are involved in the...
Keywords: Cell proliferation, Clinical, Growth factors, Ligands

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Cell Culture Troubleshooting Guide

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Peer-Reviewed Papers
15

References

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06693 Timestrip Plus -20 °C

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