T1706 Sigma-Aldrich




application(s)   CRISPR: suitable
shipped in   wet ice
storage temp.   −20°C


General description

Accomplish highly effective transfection of CRISPR/Cas9 DNA, RNA, Cas9-gRNA RNP (ribonucleoprotein) complexes and siRNA to knock-out, modify or knock-down genes of interest in a wide variety of cell types.

Features and Benefits

• Highly efficient, non-liposomal polymeric transfection/delivery method for use with MISSION CRISPR/Cas9 DNA, RNA, Cas9 RNP Complexes and siRNA.
• TransIT-CRISPR is a simple, fast, effective, and versatile. One transfection reagent to deliver CRISPR-Cas9 components in a variety of formats.
• Effective in most cell lines including difficult-to-transfect cells.


0.4ml and 1ml DNA and siRNA non-liposomal, polymeric transfection reagent


To order MISSION® CRISPR reagents, please click here

For more information and transfection protocols, please visit

Other Notes

• TransIT-CRISPR has been tested in variety of cell types including U20S and HEK 293, A549 and HeLa among others.
• It is recommended to transfect MISSION CRISPR DNA and RNA with the TransIT-CRISPR transfection reagent.
• Sigma-Aldrich is the exclusive distributor of TransIT-CRISPR from Mirus Bio LLC.

Legal Information

MISSION is a registered trademark of Sigma-Aldrich Co. LLC

TransIT-CRISPR is a registered trademark of Mirus Bio LLC

Safety & Documentation

Safety Information

UN1170 - class 3 - PG 2 - Ethanol, solution
Protocols & Articles


CRISPR/Cas Nuclease RNA-guided Genome Editing

Sign up to receive the latest updates on CRISPR technology such as new journal articles, protocols, and product launches.
Keywords: Acetylations, Catalysis, Cell culture, Degradations, Gene expression, Genetic, PAGE, Polymorphisms, Transcription, Transduction, Transfection

Efficient Delivery of Sigma-Aldrich CRISPR via a Non-Liposomal Polymeric Transfection Reagent, TransIT-CRISPR®

Efficient intracellular delivery of CRISPR/Cas9 components is needed to achieve high nuclease activity for genome editing. The potent delivery of CRISPR Cas9 plasmids, RNA and RNP (ribonucleoprotein)...
Keywords: Flow cytometry, Gene expression, Immunofluorescence, Microscopy, PAGE, Transfection

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Visualize your CRISPR Experiment with MISSION™ Cas9-GFP Fusion Proteins

Watch Cas9-GFP proteins work inside your cells and deliver the powerful nuclease editing you have come to trust and expect. MISSION™ SpCas9-GFP and eSpCas9-GFP proteins are multi-functional enzymes w...
Keywords: Flow cytometry, Transcription, Transfection

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