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T1763 Sigma-Aldrich

Trypsin Agarose

buffered aqueous suspension, from bovine pancreas trypsin

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Properties

Related Categories Application Index, Biochemicals and Reagents, Cell Dissociation, Cell Dissociation and Cell Lysis, Enzymes, Inhibitors, and Substrates,
biological source   bovine pancreas (trypsin)
form   buffered aqueous suspension
concentration   ≥15 units/mL (packed gel)
extent of labeling   ≥15 units per mL packed gel
matrix   cross-linked beaded agarose
shipped in   wet ice
storage temp.   2-8°C

Description

General description

Trypsin Agarose is an insoluble enzyme product. It is produced by reacting a conventional "soluble" enzyme (trypsin) with an inert base (agarose). This insoluble conjugate retains the activity of the original enzyme. Trypsin bound to agarose are highly stable and maintain denaturing conditions for longer time than the soluble trypsin.

Application

A very active and very stable trypsin agarose derivative has been used to optimize the design of the synthesis of a model dipeptide, benzoylarginine leucinamide. Trypsin has also been used in a study to investigate protonation-state determination in proteins using high-resolution X-ray crystallography.

Trypsin Agarose has been used for enzymatic hydrolysis of prolamins and gliadin to generate peptides.

Packaging

50 units in glass bottle

Other Notes

Insolubilized

Unit Definition

One unit will hydrolyze 1.0 μmole of BAEE per min at pH 8.0 at 30 °C (titrimetric assay).

Physical form

Suspension in approx. 10 mM acetic acid, pH 3.2

Safety & Documentation

Safety Information

Symbol 
GHS07  GHS07
Signal word 
Warning
Hazard statements 
Precautionary statements 
Target organs 
Respiratory system
RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles
Peer-Reviewed Papers
15

References

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