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T1775 Sigma-Aldrich

L-Thyroxine

powder, BioReagent, suitable for cell culture

Synonym: 3,3′,5,5″-Tetraiodo-L-thyronine, 3-[4-(4-Hydroxy-3,5-diiodophenoxy)-3,5-diiodophenyl]-L-alanine, T4

  • CAS Number 51-48-9

  • Linear Formula HOC6H2(I)2OC6H2(I)2CH2CH(NH2)CO2H

  • Molecular Weight 776.87

  •  Beilstein/REAXYS Number 2228515

  •  EC Number 200-101-1

  •  MDL number MFCD00002595

  •  eCl@ss 32190102

  •  PubChem Substance ID 24899990

  •  NACRES NA.77

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Properties

Related Categories Cell Culture, Hormones, Reagents and Supplements More...
InChI Key   XUIIKFGFIJCVMT-LBPRGKRZSA-N
product line   BioReagent
form   powder
potency   5-50 ng/mL
application(s)   cell culture | mammalian: suitable
mp   223 °C (dec.) (lit.)
Gene Information   human ... TTR(7276)
mouse ... Cnr2(12802)
rat ... Cnr1(25248)

Description

General description

Thyroxine (T4) is a tetraiodo derivative of the p-hydroxyphenyl ether of tyrosine, which functions as growth factors or cell growth inhibitors. T4 is used to treat hypothyroidism and thyroid carcinoma. It regulates energy metabolism.

Application

L-Thyroxine has been used to investigate its role in colorectal cancer cell lines HCT 116 (APC wild type) and HT-29 (APC mutant). It has also been used to evaluate its effects on pro-inflammatory tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), angiogenic vascular endothelial growth factor (VEGF), leukocyte adhesion, intercellular adhesion molecule 1 (ICAM-1) and E-selectin in human umbilical vein endothelial cells (HUVECs).

Biochem/physiol Actions

L-Thyroxine (T4) and triiodo-L-thyronine (T3) are iodine-containing hormones produced from thyroglobulin in the thyroid follicular cells. The stimulation of metabolic rate and regulation of growth and development by these hormones appear to be due to their effects on DNA transcription and, thus, protein synthesis.

Preparation Note

For long term storage, keep stock solutions at -20° in working aliquots and avoid repeated freeze/thaw cycles. Solutions stored at 2-8 °C are stable for up to 30 days.

Reconstitution

To prepare 20 μg/ml stock solution, dissolve in 50 ml sterile culture medium per mg thyroxine.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3
RTECS 
YP2833500

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles

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Peer-Reviewed Papers
15

References

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