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T1816 Sigma-Aldrich

Anti-Tumor Necrosis Factor-α antibody produced in goat

affinity isolated antibody, lyophilized powder

Synonym: Anti-TNF-α

  •  NACRES NA.41

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Apoptosis, Antibodies for Cell Biology, Antibodies to Cytokines and Growth Factors,
conjugate   unconjugated
clone   polyclonal
biological source   goat
application(s)   immunohistochemistry: 15 μg/mL
  indirect ELISA: 0.5-1.0 μg/mL
  neutralization: suitable
  western blot: 0.1-0.2 μg/mL
species reactivity   pig
form   lyophilized powder
storage temp.   −20°C
antibody form   affinity isolated antibody
Quality Level   200
antibody product type   primary antibodies
UniProt accession no.   P23563
Gene Information   pig ... TNF(397086)

Description

General description

Tumor Necrosis Factor-α (TNF-α) also refers as cachectin is a cytokine produced in response to immunological challenges such as bacteria (lipopolysaccharides), parasites, mitogens, viruses and other cytokines. It has essential roles in antitumor activity, inflammation, septic shock, immune modulation, anorexia, cachexia, hematopoiesis and viral replication. It also facilitates the growth of human fibroblasts, collagenase production as well as can activates polymorphonuclear neutrophils and osteoclasts. Anti-Tumor Necrosis Factor-α antibody can be used in neutralization and immunoblotting (working concentration-0.1-0.2 μg/ml) for detecting porcine TNF-α. Goat anti-Tumor Necrosis Factor-α antibody reacts specifically with recombinant porcine tumor necrosis factor α.

Specificity

The antibody neutralizes the biological activity of recombinant porcine TNF-α.

Immunogen

recombinant porcine TNF-α expressed in Escherichia coli.

Application

Anti-Tumor Necrosis Factor-α antibody can be used in immunohistochemistry and indirect ELISA.

Physical form

Lyophilized from a 0.2 μm filtered solution of phosphate buffered saline.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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