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T1827 Sigma-Aldrich

Monoclonal Anti-TBP antibody produced in mouse

~2.0 mg/mL, clone 58C9, purified immunoglobulin, buffered aqueous solution

Synonym: Anti-GTF2D, Anti-GTF2DI, Anti-SCA17, Anti-TATA box binding protein, Anti-TFIID

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Epigenetics and Nuclear Signaling, Antibodies for Gene Regulation,
conjugate   unconjugated
clone   58C9, monoclonal
biological source   mouse
application(s)   immunoprecipitation (IP): suitable
  western blot: 1-2 μg/mL using nuclear extracts of D.Mel cells
species reactivity   Drosophila melanogaster, yeast, Sf9 cell line, human
mol wt   antigen ~42 kDa
form   buffered aqueous solution
shipped in   dry ice
storage temp.   −20°C
antibody form   purified immunoglobulin
isotype   IgG2b
antibody product type   primary antibodies
concentration   ~2.0 mg/mL
UniProt accession no.   P20226
Gene Information   Drosophila melanogaster ... Tbp(37476)
human ... TBP(6908)

Description

General description

TBP (TATA-binding protein) is encoded by the gene mapped to human chromosome 6q27. The encoded protein is a key constituent of RNA polymerase II transcription apparatus in eukaryotic cells.

Immunogen

Drosophila TFIID complex.

Target description

TBP encodes TBP, the TATA-binding protein. A distinctive feature of TBP is a long string of glutamines in the N-terminus. This region of the protein modulates the DNA binding activity of the C terminus, and modulation of DNA binding affects the rate of tr

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

The TBP (TATA-binding protein) gene encodes a subunit of TFIIIB (transcription factor IIIB) complex and recognizes the TATA box in the DNA. Thus, it plays an important role in RNA polymerase II transcription machinery. TBP is known to induce stress granule formation in hepatitis B viral infection. Mutation in the gene is associated with the development of spinocerebellar ataxia 17 (SCA17).

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

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Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
How do I find price and availability?
There are several ways to find pricing and availability for our products.Once you log onto our website, you will find the price and availability displayed on the product detail page.You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 orview local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
Which light chain is used in Product T1827,Monoclonal Anti-TBP antibody, clone 58C9?
The light chain for Product T1827,Monoclonal Anti-TBP antibody, clone 58C9 is a lambda light chain.
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Protocols & Articles

Articles

Antibody Basics

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Keywords: Apoptosis, Huntington Disease, Transcription

Protocols

Western Blot Protocol | Immunoblotting Protocol

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Keywords: AGE, Buffers, Cell disruption, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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