T1895 Sigma-Aldrich


powder, BioReagent, suitable for cell culture

Synonym: 1-(2-Deoxy-β-D-ribofuranosyl)-5-methyluracil, 1-(2-Deoxy-β-D-ribofuranosyl)thymine, 2′-Deoxythymidine, Thymine deoxyriboside, dT

  • CAS Number 50-89-5

  • Empirical Formula (Hill Notation) C10H14N2O5

  • Molecular Weight 242.23

  •  Beilstein/REAXYS Number 89285

  •  EC Number 200-070-4

  •  MDL number MFCD00006537

  •  PubChem Substance ID 24899996

  •  NACRES NA.75



Related Categories Cell Culture, Conditioned media supplements and reagents, Hybridoma Reagents, ITS and Other Supplements, Miscellaneous Reagents and Supplements,
Quality Level   200
product line   BioReagent
assay   ≥99%
form   powder
application(s)   cell culture | mammalian: suitable
mp   186-188 °C (lit.)
SMILES string   CC1=CN([C@H]2C[C@H](O)[C@@H](CO)O2)C(=O)NC1=O
InChI   1S/C10H14N2O5/c1-5-3-12(10(16)11-9(5)15)8-2-6(14)7(4-13)17-8/h3,6-8,13-14H,2,4H2,1H3,(H,11,15,16)/t6-,7+,8+/m0/s1
Gene Information   human ... DTYMK(1841)


General description

Thymidine is also referred to as pyrimidine deoxynucleoside. Deoxythymidine is a nucleoside present in DNA. In a DNA double stranded structure, thymidine pairs with deoxyadeninosine.


Thymidine can be used in HT and HAT cocktails for hybridoma fusion, selection and cloning. It has been used:
• as a nucleotide source in minimal essential medium to evaluate the outgrowth of the iSCNT-derived blastocysts
• as a supplement in Dulbecco′s Modified Eagle Medium (DMEM) selection medium used for the production of anti-neurogranin (NRGN) antibodies
• to prepare THMG (thymidine, hypoxanthine, glycine, methotrexate) and THG stock solution for cleansing mouse lymphoma cells


1, 5, 10, 25 g in poly bottle

Biochem/physiol Actions

Thymidine is useful in cell synchronization during S-phase. In the salvage pathway of pyrimidines, pyrimidine phosphorylase reversibly converts thymine to thymidine.

Safety & Documentation

Safety Information

Personal Protective Equipment 
NONH for all modes of transport
WGK Germany 


Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
How can I solubilize Product T1895, Thymidine?
This product is soluble in water (50 mg/mL), yielding a clear, colorless solution with heating. For aqueous concentrations higher than 50 mg/mL, solubility can be enhanced with the addition of a small amount of NaOH solution. Add the base drop wise with slow stirring. Thymidine is also soluble in methanol, hot alcohol, hot acetone, hot ethyl acetate, pyridine, glacial acetic acid, and is sparingly soluble in hot chloroform.
What concentration of Product T1895, Thymidine,is used for testing this product for cell culture applications?
The tested working concentration for cell culture for Thymidine is 8 mg/L.
How can I sterilize a solution of Product T1895, Thymidine?
Solutions can be sterile filtered using 0.2 μm filters (low protein binding). Do not autoclave solutions.
How long can I store media supplemented with Product T1895, Thymidine?
Thymidine is a component of Nutrient Mixture F-12 at 0.73mg/L.This media has an expiration date of 18 months from date of formulation.You will need to determine if this is appropriate for your application.
How do I find price and availability?
There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 or view local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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