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T1952 Sigma-Aldrich

Trichostatin A, Ready Made Solution

5 mM in DMSO (0.2 μm-filtered), from Streptomyces sp.

Synonym: TSA, [R-(E,E)]-7-[4-(Dimethylamino)phenyl]-N-hydroxy-4,6-dimethyl-7-oxo-2,4-heptadienamide

  • CAS Number 58880-19-6

  • Empirical Formula (Hill Notation) C17H22N2O3

  • Molecular Weight 302.37

  •  NACRES NA.32

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Properties

Related Categories Antibiotic Ready Made Solutions, Antibiotics, Bioactive Small Molecules for Epigenetic Research, Biochemicals and Reagents, Cell Biology,
Quality Level   200
biological source   Streptomyces sp.
form   DMSO solution
concentration   5 mM in DMSO (0.2 μm-filtered)
Mode of action   enzyme | inhibits
antibiotic activity spectrum   fungi
  neoplastics
shipped in   dry ice
storage temp.   −20°C

Description

General description

Trichostatin A is a compound of primary hydroxamic acid.

Application

Trichostatin A, ready-made solution has been used:
• as a histone deacetylase inhibitor to study its effect on transcriptome changes by stem cell testing
• to inhibit histone deacetylase (HDAC) class I, II, or III in primary pituitary cell cultures and to investigate insulin control of endogenous human growth hormone gene (hGH)
• to treat cells for the HDAC inhibition experiments

Biochem/physiol Actions

Trichostatin A (TSA) is a Streptomyces metabolite, which specifically inhibits mammalian histone deacetylase at a nanomolar concentration and causes accumulation of highly acetylated histone molecules in mammalian cells. For that reason, trichostatin A is a tool to study the consequences of histone acetylation in vivo. Trichostatin A induces cell differentiation, cell cycle arrest, reversal of transformed cells morphology, and apoptosis and is able to modulate transcription. TSA has been used to establish a new cloning technique, which increases the success rates for mouse cloning.

Safety & Documentation

Safety Information

RIDADR 
NA 1993 / PGIII
WGK Germany 
1

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles
Peer-Reviewed Papers
15

References

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T8552 Trichostatin A, ≥98% (HPLC), from Streptomyces sp.
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EPI006 Histone Deacetylase 8 (HDAC8) Activity Assay Kit, 100 assays in 96 well plates
EPI007 Histone Deacetylase 8 (HDAC8) Inhibitor Screening Kit, 100 assays in 96 well plates
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92210 Timestrip Plus 0 °C
06693 Timestrip Plus -20 °C

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