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T2076 Sigma

TargeTron Vector pAR1219

Expression Vector for Bacterial Gene Knockout

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Related Categories Functional Genomics and RNAi, Molecular Biology, TargeTron Gene Knock Out System More...
shipped in   wet ice
storage temp.   −20°C

Description

General description

Plasmid pAR1219 is a pBR322-based vector that expresses T7 RNA Polymerase under control of the IPTG inducible lacUV5 promoter and is intended for use with the TargeTron Gene Knockout System (TA0100).

Other Notes

For more information and to view applications data, please visit www.sigma-aldrich.com/targetron.

Packaging

2 μg in poly bottle

Preparation Note

To use pAR1219 in conjunction with the pACD4 plasmids, simply co-transform both plasmids and select in a liquid medium containing: 50 mg/ml ampicillin, 25 mg/ml chloramphenicol, and 1% glucose. Glucose is typically included to provide additional suppression of the lac UV5 promoter prior to IPTG-induction.

Application

Many TargeTron system plasmids use the T7 promoter for intron expression. By co-transforming plasmid pAR1219 with the TargeTron pACD4 plasmids, the T7 promoter can be used to express the intron and disrupt chromosomal genes in alternative hosts such as Salmonella typhimurium and Shigella flexneri. Chromosomal gene disruptions in non-DE3 strains of E. coli can also be performed using pAR1219 with the pACD4 intron expression plasmids. TargeTron Vector pAR1219 has been used for the overexpression of T7 RNA polymerase in the preparation of S30-T7 lysate. It has also been used to transform MRE600 E. coli strains.

Legal Information

TargeTron is a trademark of InGex, LLC

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3
Protocols & Articles

Related Content

TargeTron® Gene Knockout System Animation [VIDEO]

The TargeTron Gene Knockout System provides optimized reagents and protocols for the rapid and specific disruption of bacterial genes by insertion of group II introns. Unlike conventional DNA transpo...
Keywords: Genetic

Peer-Reviewed Papers
15

References

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