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  • T2190 - Anti-Tumor Necrosis Factor Soluble Receptor I antibody produced in goat

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T2190 Sigma-Aldrich

Anti-Tumor Necrosis Factor Soluble Receptor I antibody produced in goat

affinity isolated antibody, lyophilized powder

Synonym: Anti-TNFsRI

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Apoptosis, Antibodies for Cell Biology, Antibodies for Hematopoietic Stem Cells,
conjugate   unconjugated
clone   polyclonal
biological source   goat
application(s)   flow cytometry: suitable
  immunohistochemistry (frozen sections): suitable
  western blot: suitable
species reactivity   mouse
form   lyophilized powder
storage temp.   −20°C
antibody form   affinity isolated antibody
Quality Level   100
antibody product type   primary antibodies
UniProt accession no.   P25118
Gene Information   mouse ... Tnfrsf1a(21937)

Description

General description

The antibody recognizes cell surface mouse TNF RI (55 kDa) and exhibits TNF agonist activities. The antibody shows < 5% cross-reactivity with recombinant human TNF sRI and no cross-reactivity with recombinant human TNFsRII.

Immunogen

recombinant mouse TNF soluble receptor I, expressed in Escherichia coli.

Physical form

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing 5% trehalose.

Biochem/physiol Actions

The biological effects of tumor necrosis factor soluble factor (TNSF) are mediated by either type I or type II receptors. Type I receptors that are ubiquitously present on all cells whereas type II receptors are expressed only on cells of immune system and endothelial cells. Receptors of TNFSF such as TNFR1 and Fas have their own death domains that activate the apoptosis. Other receptors such as TNFR2 do not have death domains and utilize TNF associated factors (TRAFs) to activate intracellular signaling. The pathways activated via TNFsRI include NF-κB, JNK and MAPK.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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