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T2194 Sigma-Aldrich

Trizma® hydrochloride solution

pH 7.4, BioPerformance Certified, 1 M, suitable for cell culture

Synonym: Tris hydrochloride solution

  •  NACRES NA.25

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Properties

Related Categories BioPerformance Certified Buffers, Biochemicals and Reagents, Biological Buffers, Buffer Convenience Packaging, Buffer Solutions,
Quality Level   200
grade   BioPerformance Certified
  for molecular biology
form   solution
concentration   1 M
application(s)   cell culture | mammalian: suitable
impurities   DNase, RNase, Protease, none detected
  bioburden, tested
  endotoxin, tested
  ≤5 ppm Heavy metals (as Pb)
pH   7.4
useful pH range   7.0 - 9.0
absorption   ≤0.05 at 290 at 40%

Description

General description

A series of Pre-mixed solutions of TRIZMA Base and TRIZMA HCl to provide commonly used pH values for Tris buffers. No mixing or pH adjustment necessary. Guaranteed accuracy ± 0.1 pH units.

Application

Trizma®hydrochloride solution has been used:
• in the preparation of cell-lysis buffer
• as a component of wash buffers and RNase mix 1, used in the preparation of 5′-complete cDNAs for paired-end sequencing
• in dissolving cadmium or S2- loaded microreactors for CdS synthesis

Legal Information

Trizma is a registered trademark of Sigma-Aldrich Co. LLC

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 2
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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pHast Pack
Protocols & Articles

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Peer-Reviewed Papers
15

References

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