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T2200 Sigma-Aldrich

Anti-β-Tubulin III antibody produced in rabbit

Antibody Enhanced Validation

affinity isolated antibody, buffered aqueous solution

Synonym: Anti-Tuj 1

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies against Proteins/Bioactives/Markers/Receptors for Stem Cell Biology, Antibodies for Cell Biology, Antibodies for Neural Stem Cells,
conjugate   unconjugated
clone   polyclonal
biological source   rabbit
application(s)   immunoprecipitation (IP): 10 μg using RIPA extract (250 μg) of cultured rat PC12 cells
  indirect immunofluorescence: 10-20 μg/mL using rat PC12 cells
  western blot: 0.2-0.4 μg/mL using whole extracts of mouse brain or cultured human neuroblastoma SH-SY5Y cells
species reactivity   human, rat, mouse
mol wt   antigen mol wt ~55 kDa
enhanced validation   knockout
Learn more about Antibody Enhanced Validation
form   buffered aqueous solution
shipped in   dry ice
storage temp.   −20°C
antibody form   affinity isolated antibody
antibody product type   primary antibodies
UniProt accession no.   Q13509
Gene Information   human ... TUBB3(10381)
mouse ... Tubb3(22152)
rat ... Tubb3(246118)

Description

General description

Tubulin β 3 class III (TUBB3) also known as β-Tubulin III, is encoded by the gene mapped to human chromosome 16q24.3. TUBB3 protein expression is restricted to neurons.
α/β-Tubulin, an integral component of microtubules, is present in almost all eukaryotic cells.
Anti-β-Tubulin III is developed in rabbit using as immunogen a synthetic peptide corresponding to amino acid residues 441-450 of human β-tubulin III (Ala 446 to Ser446 substitution) with N-terminal added cysteine, conjugated to KLH. The sequence is conserved in mammals. α/β-Tubulin occurs mostly as soluble (approx. 100-110 kDa) heterodimeric sets of α- and β-tubulin isotypes or as polymers in assembled microtubules. β-Tubulin III (also designated β-4 chain) is found in the brain and dorsal root ganglia and appears to be localized to neurons of the central and peripheral nervous system. β-tubulin III is also found in Sertoli cells of the testis, spermatozoa tails, certain lung cells, and apparently some breast stromal cells.

Specificity

Anti-β-Tubulin III recognizes human, mouse,and rat β-tubulin III.

Immunogen

synthetic peptide corresponding to amino acid residues 441-450 of human β-tubulin III (Ala446 to Ser446 substitution) with N-terminal added cysteine, conjugated to KLH. The sequence is conserved in mammals.

Application

Anti-β-Tubulin III antibody produced in rabbit has been used in following studies:
• Immunofluorescence.
• Immunostaining.
• Western blotting.

Anti-β-Tubulin III antibody produced in rabbit has been used in following studies:
• Immunofluorescence
• Immunostaining
• Western blotting
• Indirect immunoperoxidase assay (IPA)

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Preparation Note

Storage and Stability: For continuous use, store at 2-8 °C for up to one month.
For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. Storage in frost-free freezers is also not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Biochem/physiol Actions

Tubulin β 3 class III (TUBB3) plays a vital role in nervous system development and axon guidance. Mutation of the gene encoding protein leads to the ocular motility disorder, congenital fibrosis of the extraocular muscle type 3 (CFEOM3), and various neurological syndromes. Altered expression of the protein affects microtubule dynamics and microtubule-kinesin interactions. Increased expression of TUBB3 is observed in tumor tissues. Decreased expression of TUBB3 can be considered as an important marker for poor prognosis of cutaneous malignant melanoma. TUBB3 is implicated in the suppression of invasive growth of tumor tissue;therefore, it is considered to be a potential target for development of antitumor drugs.
TUBB4 is responsible for microtubule mediated N-cadherin transport to the cell membrane, which is necessary for cell-cell contact formation. TUBB4 participates in the progression of osteosarcoma.
Microtubules function as structural and mobile elements in mitosis, intracellular transport, flagellar movement, cell morphogenesis, and other cytoskeletal functions. Increased β-Tubulin III expression is observed during axonal growth. β-tubulin III is also found in certain tumors of non-neural origin, such as lymphoma, squamous cell carcinoma, giant cell glioblastoma multiforme, oligodendroglioma, and malignant melanoma but not in their non-neoplastic adult counterparts. Its expression is increased in various chemoresistant epithelial cell lines and gliomas and lung cancer with ascending grade of malignancy.

Other Notes

In order to obtain the best results using various techniques and preparations, we recommend determining the optimal working concentrations by titration.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Milli-Q® Water Purification Solutions
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Chondroitinase for Neural Regeneration Research

The spinal cord is the information highway system for the brain. And axons are the road that allows information data to be transferred. So, when a traumatic spinal cord injury (SCI) occurs and axons ...
Keywords: Catalysis, Cell culture, Degradations, High performance liquid chromatography, Immunofluorescence, PAGE

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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T3952 Anti-β-Tubulin III antibody produced in rabbit, ~1 mg/mL, affinity isolated antibody, buffered aqueous solution

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