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  • T2315 - Anti-Tumor Necrosis Factor Soluble Receptor Type II antibody produced in goat

T2315 Sigma-Aldrich

Anti-Tumor Necrosis Factor Soluble Receptor Type II antibody produced in goat

IgG fraction of antiserum, lyophilized powder

Synonym: CD120b antigen, Etanercept, TNF sRII, TNF-R2, p75 TNF receptor, p75TBPII, p75TNFR, tumor necrosis factor receptor superfamily member 1B



Related Categories Alphabetical Index, Antibodies, Antibodies for Apoptosis, Antibodies for Cell Biology, Antibodies for Hematopoietic Stem Cells,
conjugate   unconjugated
clone   polyclonal
biological source   goat
application(s)   immunocytochemistry: 5-15 μg/mL (detects TNF RII in human PBLs)
  western blot: 1 μg/mL (detects rhs TNF RII at 5 ng/lane under non-reducing conditions)
species reactivity   human
form   lyophilized powder
storage temp.   −20°C
antibody form   IgG fraction of antiserum
Quality Level   200
antibody product type   primary antibodies
UniProt accession no.   P20333
Gene Information   human ... TNFRSF1B(7133)


General description

TNF RII is a type I transmembrane glycoprotein primarily expressed on hematopoietic cells and is designated TNFRSF1B. TNF RII is a highaffinity receptor for TNF alpha and TNF beta. A soluble form of the receptor generated by proteolytic cleavage functions as a TNF antagonist.


The antibody detects human TNF sRII and shows no cross-reactivity with recombinant human TNF sRI.


recombinant human TNF soluble receptor II (TNF sRII) expressed in Escherichia coli. The immunogen is the non-glycosylated, N-terminal methionyl form of the naturally-occurring human soluble type II receptor for TNF, minus 53 amino acids from the proline-rich region just exterior to the transmembrane domain.


Western Blot: 1 ug/mL detects rhs TNF RII at 5 ng/lane under non-reducing conditions
Immunocytochemistry: Concentration of 5-15 ug/ml detects TNF RII in human peripheral blood lymphocytes

Optimal dilutions should be determined by the end user.

Physical form

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline (pH 7.4) and 5% trehalose.


Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Other Notes

Protein A or G purified

Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable


Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles


Antibody Basics

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Western Blot Protocol | Immunoblotting Protocol

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