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T2391 Sigma-Aldrich

Triosephosphate Isomerase from rabbit muscle

Type III-S, ammonium sulfate suspension, ≥4,000 units/mg protein

Synonym: D-Glyceraldehyde-3-phosphate ketol-isomerase, TPI

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Properties

Related Categories 5.3.x.x Intramolecular oxidoreductases, 5.x.x.x Isomerases, Biochemicals and Reagents, Cell Biology, Enzyme Class Index,
Quality Level   200
type   Type III-S
form   ammonium sulfate suspension
mol wt   calculated mol wt 53.2 kDa
foreign activity   3-phosphoglyceric phosphokinase ≤0.01%
  glyceraldehyde-3-phosphate dehydrogenase ≤0.01%
  pyruvate kinase, lactic dehydrogenase, phosphoglucose isomerase, α-glycerophosphate dehydrogenase and aldolase ≤0.001%
storage temp.   2-8°C

Description

Application

Triosephosphate isomerase has been used in a study to assess molecular characterizations of Cryptosporidium, Giardia, and Enterocytozoon. Triosephosphate isomerase has also been used in a study to investigate apoptosis of hepatocellular carcinoma cell lines.

Unit Definition

One unit will convert 1.0 μmole D-glyceraldehyde 3-phosphate to dihydroxyacetone phosphate per min at pH 7.6 at 25 °C.

Physical form

Crystalline suspension in 3.2 M (NH4)2SO4 solution, pH 6.0

Analysis Note

Protein determined by biuret

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 2
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles

Articles

Ammonium Sulfate Suspensions - Technical Note

Note that for an ammonium sulfate suspension, most of the enzyme will be in solid form. It is likely that only negligible amounts of enzyme will be in the ammonium sulfate solution.
Keywords: Enzyme activity

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Peer-Reviewed Papers
15

References

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