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T2705 Sigma-Aldrich

Topotecan hydrochloride hydrate

≥98% (HPLC and enzymatic)

Synonym: 9-[(dimethylamino)methyl]-10-hydroxy-(20S)-camptothecin hydrochloride hydrate, NSC-609669 hydrochloride hydrate, SKF-104864A hydrochloride hydrate, hycamptamine hydrochloride hydrate

  • CAS Number 123948-87-8 (free base)

  • Empirical Formula (Hill Notation) C23H23N3O5 · xHCl · yH2O

  • Molecular Weight 421.45 (anhydrous free base basis)

  •  MDL number MFCD00870670

  •  PubChem Substance ID 329826724

  •  NACRES NA.77



Related Categories Apoptosis and Cell Cycle, Approved Therapeutics/Drug Candidates, Bioactive Small Molecule Alphabetical Index, Bioactive Small Molecules, Cell Biology,
assay   ≥98% (HPLC and enzymatic)
form   powder
storage condition   desiccated
  protect from light
color   yellow
solubility   DMSO: ≥20 mg/mL
originator   GlaxoSmithKline
storage temp.   −20°C



Topotecan has been used as a positive control for the identification and analysis of HIF-1α and VEGF inhibitors in human glioma cells under hypoxic conditions1. It has also been used for in vitro apoptosis assays in PA317 cells2.


10, 50 mg in glass bottle

Biochem/physiol Actions

Topotecan is a topoisomerase I inhibitor and an apoptosis inducer. It is a potent antineoplastic agent.

Features and Benefits

This compound is a featured product for ADME Tox and Apoptosis research. Discover more featured ADME Tox and Apoptosis products. Learn more about bioactive small molecules for other areas of research at sigma.com/discover-bsm.

This compound is also offered as part of Sigma′s Library of Pharmacologically Active Compounds (LOPAC®1280), a biologically annotated collection of high-quality, ready-to-screen compounds. Click here to learn more.

This compound was developed by GlaxoSmithKline. To browse the list of other pharma-developed compounds and Approved Drugs/Drug Candidates, click here.

Preparation Note

Topotecan hydrochloride hydrate is soluble in DMSO at a concentration that is greater than or equal to 20 mg/ml.

Legal Information

LOPAC is a registered trademark of Sigma-Aldrich Co. LLC

Safety & Documentation

Safety Information

GHS08  GHS08
Signal word 
Hazard statements 
Precautionary statements 
NONH for all modes of transport
WGK Germany 


Certificate of Analysis (COA)

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Protocols & Articles


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