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T3038 Sigma-Aldrich

Trizma® hydrochloride solution

1 M, BioReagent, for molecular biology

Synonym: Tris hydrochloride solution



Related Categories Biochemicals, Biochemicals and Reagents, Biological Buffers, Buffer Convenience Packaging, Buffer Solutions,
grade   for molecular biology
  for molecular biology
sterility   0.2 μm filtered
product line   BioReagent
concentration   1 M
impurities   DNase, RNase, NICKase and protease, none detected
pH   8.0
Featured Industry   Agriculture


General description

Trizma® hydrochloride solution is a useful biological buffer.


Trizma® hydrochloride solution may be used in the following studies:
• As buffer for the 2-D electrophoresis of rat fibroblast cell.
• As buffer for the rapid isolation of high molecular weight plant DNA (50,000 base pairs or more in length).
• Selective immunoprecipitation of biotin-labeled DNA with antibiotin IgG and Staphylococcus sp.

The pH values of all buffers are temperature and concentration dependent. For Tris buffers, pH increases about 0.03 unit per degree C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.
For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.

Other Notes

Prepared with pH-adjusted Biotechnology Performance Certified Trizma Base in 18 megohm water and 0.2 μm filtered.

Legal Information

Trizma is a registered trademark of Sigma-Aldrich Co. LLC

Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 


Certificate of Analysis


Certificate of Origin


Milli-Q® Water Purification Solutions
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