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T3038 Sigma-Aldrich

Trizma® hydrochloride solution

1 M, BioReagent, for molecular biology

Synonym: Tris hydrochloride solution

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Properties

Related Categories Biochemicals, Biochemicals and Reagents, Biological Buffers, Buffer Convenience Packaging, Buffer Solutions,
grade   for molecular biology
  for molecular biology
InChI Key   QKNYBSVHEMOAJP-UHFFFAOYSA-N
sterility   0.2 μm filtered
product line   BioReagent
concentration   1 M
impurities   DNase, RNase, NICKase and protease, none detected
pH   8.0
Featured Industry   Agriculture

Description

General description

Trizma® hydrochloride solution is a useful biological buffer.

Application

Trizma® hydrochloride solution may be used in the following studies:
• As buffer for the 2-D electrophoresis of rat fibroblast cell.
• As buffer for the rapid isolation of high molecular weight plant DNA (50,000 base pairs or more in length).
• Selective immunoprecipitation of biotin-labeled DNA with antibiotin IgG and Staphylococcus sp.

The pH values of all buffers are temperature and concentration dependent. For Tris buffers, pH increases about 0.03 unit per degree C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.
For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.

Other Notes

Prepared with pH-adjusted Biotechnology Performance Certified Trizma Base in 18 megohm water and 0.2 μm filtered.

Legal Information

Trizma is a registered trademark of Sigma-Aldrich Co. LLC

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
2

Documents

Certificate of Analysis

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Certificate of Origin

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Milli-Q® Water Purification Solutions
Protocols & Articles

Protocols

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Peer-Reviewed Papers
15

References

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