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T3253 Sigma-Aldrich

Trizma® hydrochloride

reagent grade, ≥99.0% (titration), crystalline

Synonym: TRIS HCl, TRIS hydrochloride, Tris(hydroxymethyl)aminomethane hydrochloride, Tromethane hydrochloride

  • CAS Number 1185-53-1

  • Linear Formula NH2C(CH2OH)3 · HCl

  • Molecular Weight 157.60

  •  Beilstein/REAXYS Number 3675235

  •  EC Number 214-684-5

  •  MDL number MFCD00012590

  •  PubChem Substance ID 24900119

  •  NACRES NA.25

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Properties

Related Categories Apoptosis and Cell Cycle, Biochemicals and Reagents, Biological Buffers, Buffers, Buffers A to Z,
Quality Level   400
grade   reagent grade
assay   ≥99.0% (titration)
form   crystalline
impurities   ≤0.5% water (Karl Fischer)
useful pH range   7.0 - 9.0
pKa (25 °C)   8.1
mp   150-152 °C
solubility   H2O: soluble, clear, colorless
absorption   ≤0.05 at 290 nm at 40%
Featured Industry   Diagnostic Assay Manufacturing
SMILES string   Cl.NC(CO)(CO)CO
InChI   1S/C4H11NO3.ClH/c5-4(1-6,2-7)3-8;/h6-8H,1-3,5H2;1H
InChI key   QKNYBSVHEMOAJP-UHFFFAOYSA-N

Description

General description

Trizma® hydrochloride is mainly required for preparation of buffer at physiological range of 7.3 to 7.5. The prepared buffer is compatible with biological fluids. It is of importance to laboratories as a standard pH solution.

Application

The pH values of all buffers are temperature and concentration dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.
For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.

Trizma® hydrochloride has been used as a buffer solution for staining procedure, reduction and alkylation studies and in situ hybridization procedure.­

Packaging

1 kg in poly bottle

100, 250, 500 g in poly bottle

5, 10, 25, 50 kg in poly drum

Other Notes

Easily compare specifications for Trizma HCl products with the Trizma HCl specification table.

Legal Information

Trizma is a registered trademark of Sigma-Aldrich Co. LLC

Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 1
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
What is the pH of Trizma HCl, Product T3253,in solution?
The pH of Trizma hydrochloride in water is dependent on the concentration. The pH of a 0.1 M aqueous solution is approximately 4.7 while the pH of a 40% (w/w) aqueous solution is typically 2.6-3.3.
How can I find the technical bulletin 106B that is referenced in the Product Information Sheet for Product T3253,Trizma® hydrochloride?
Under the Documents section above.
Is Product T3253,Trizma® hydrochloride, the same as Tris buffer?
No. Trizma HCl in solution at 0.05 M will produce a pH of approximately 4.7, but will have little if any buffering capacity. Trizma Base in solution at 0.05 M will produce a pH of approximately 10.4, but will have little if any buffering capacity. Blending Trizma Base and Trizma HCl will produce any desired pH between 7 and 9, with a reasonable buffering capacity.
How do I find price and availability?
There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 or view local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
My question is not addressed here, how can I contact Technical Service for assistance?
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Protocols & Articles

Protocols

Hand-casting gels for PAGE and SDS-PAGE using TurboMix™ Bis-Tris Gel Casting Kits

Polyacrylamide gel electrophoresis (PAGE) is a foundational technique used to separate proteins and other macromolecules by their electrophoretic mobility. Polyacrylamide gels are formed through the ...
Keywords: Alkylations, Cell disruption, Deaminations, Degradations, Electrophoresis, Gel electrophoresis, Nucleic acid denaturation, PAGE, Polymerization reactions, Sample preparations, Separation, Titrations

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Peer-Reviewed Papers
15

References

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