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T3824 Sigma-Aldrich

Tyrosinase from mushroom

lyophilized powder, ≥1000 unit/mg solid

Synonym: Catechol Oxidase, Monophenol Monooxygenase, Monophenol, dihydroxyphenylalanine:oxygen oxidoreductase, Polyphenol Oxidase

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Properties

Related Categories 1.14.x.x Acting on paired donors with incorporation or reduction of molecular oxygen, 1.x.x.x Oxidoreductases, Application Index, Biochemicals and Reagents, Cell Biology,
Quality Level   200
form   lyophilized powder
mol wt   119.5 kDa by electrophoresis
  128 kDa by sedimentation velocity diffusion
  133 kDa by LS
Featured Industry   Diagnostic Assay Manufacturing
shipped in   wet ice
storage temp.   −20°C

Description

General description

Isoelectric point (pI): 4.7-5
pH optimum is 6-7

Molecular weight: 128 kDa by sedimentation velocity diffusion; 133 kDa by light-scattering measurements, and 119.5 kDa by electrophoresis.

Tyrosinase is a copper-containing oxidase, which has activity for both catechols and cresol. It is responsible for browning reactions. The enzyme is reported to have two binding sites for aromatic substrates and a different binding site for oxygen-copper. The copper is probably Cu(I), with inactivation involving oxidation to Cu(II) ion.

Tyrosinase is a copper-containing oxidase with activity for catechols and cresol. Tyrosinase participates in the conversion of tyrosine to dihydroxyphenylalanine (DOPA), and polymerization of DOPA leads to melanin formation. Tyrosinase has a role in browning reactions. The enzyme is said to have two binding sites for aromatic substrates and a distinct binding site for oxygen-copper.

Application

Tyrosinase activity has been assessed in a study that developed an alternative therapeutic agent for treating hyperpigmentation. Tyrosinase has also been used in a study to investigate the oculocutaneous albinism phenotype in the Pakistani population.

Packaging

25000, 50000 units in glass bottle

250000, 500000, 1000000 units in poly bottle

Biochem/physiol Actions

Converts tyrosine to L-DOPA or tyramine to dopamine

Physical properties

Isoelectric point (pI): 4.7-5
pH optimum is 6-7

Molecular weight: 128 kDa by sedimentation velocity diffusion; 133 kDa by light-scattering measurements, and 119.5 kDa by electrophoresis.

Unit Definition

One unit = ΔA280 of 0.001 per min at pH 6.5 at 25 °C in 3 mL reaction mix containing L-tyrosine.

Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

Please Enter a Lot Number

Certificate of Origin (COO)

Please Enter a Lot Number

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
How can Tyrosinase from mushroom, Product T3824, be dissolved and what is its expected stability in solution?
Tyrosinase can be dissolved in 50 mM potassium phosphate buffer at 2 mg/mL.Solutions are expected to retain activity for several days in the refrigerator (2-8°C) and for several weeks in the freezer (-20°C).
The website says Product T3824, Tyrosinase from mushroom, should be stored at -20°C, but I received it at room temperature. Is this correct?
The product is shipped at ambient temperature and is stable for up to two weeks during shipment.Once received, it is recommended to store the product at -20 deg. C to insure the quality for long term storage.
I see that the approximate activity of Product T3824, Tyrosinase from mushroom,is?1000 unit/mg solid, but how can I find the lot specific activity of this tyrosinase?
The certificate of analysis for the lot will report the exact tyrosinase activity for a particular batch. The lot number can be found on the label of the bottle, or you can call technical service (800-325-5832) for a sample lot number to view the online certificate of analysis.
I used to order another tyrosinase (Product No. T7755), which has since been discontinued. What is the difference between the replacement product T3824 and the discontinued product T7755?
The difference between these two products is that T3824 has been stabilized with trehalose, while T7755 was not.
How do I find price and availability?
There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 or view local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
My question is not addressed here, how can I contact Technical Service for assistance?
Ask a Scientist here.
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