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T3913 Sigma-Aldrich

Tris-Borate-EDTA buffer

5× concentrate, powder blend

Synonym: TBE buffer

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Properties

Related Categories Biochemicals and Reagents, Biological Buffers, Buffer Convenience Packaging, Buffers A to Z, Molecular Biology,
Quality Level   200
form   powder blend
impurities   DNase, RNase and NICKase, none detected
pH   8.1-8.5 (25 °C, 5 ×)
solubility   water: 85.1 g/L, clear, colorless
suitability   suitable for gel electrophoresis (after dilution to working concentration)
Featured Industry   Diagnostic Assay Manufacturing
SMILES string   OB(O)O.NC(CO)(CO)CO.OC(=O)CN(CCN(CC(O)=O)CC(O)=O)CC(O)=O
InChI   1S/C10H16N2O8.C4H11NO3.BH3O3/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;5-4(1-6,2-7)3-8;2-1(3)4/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);6-8H,1-3,5H2;2-4H
InChI key   OSBLTNPMIGYQGY-UHFFFAOYSA-N

Description

Application

Tris-Borate-EDTA buffer is suitable for use in gel electrophoresis.
It may be employed for the following studies:
• As buffer system for the evaluation of molecular weights of glycoproteins of ovalbumin, avidin and fetuin by sodium dodecyl sulfate-pore gradient electrophoresis.
• For the hemoglobin electrophoresis in starch gel.
• Preparation of buffer concentration gradient gel for the rapid determination of DNA sequence.

TBE running buffer is the most commonly used buffer for DNA and RNA polyacrylamide gel electrophoresis. TBE is used with non-denaturing or denaturing (7 M urea) gels. It is also routinely used for DNA automated sequencing gel. TBE can also be used for agarose gels but is not recommended for preparative gels for recovery of nucleic acids.
Dilution of the TBE stock concentrates to a 1× TBE running buffer results in a buffer containing 89 mM Tris-borate and 2 mM EDTA, pH 8.3. The 5× or 10× stocks may also be added to an acrylamide/bis-acrylamide stock solution for making the PAGE gel. Applied voltages of less than 5 V/cm (distance between the electrodes of the unit) are recommended for maximum resolution.

Packaging

Packaged in pouches

Preparation Note

Prepared with Biotechnology Performance Certified Trizma base (Product Code T6066) and Molecular Biology Reagents boric acid (Product Code B6768) and EDTA disodium salt (Product Code E5134).

Reconstitution

Produces a 5× concentrate (0.445 M Tris-borate, 10 mM EDTA, pH 8.3) after dissolving with the indicated amount of water. A suitable container must be supplied.

Safety & Documentation

Safety Information

Symbol 
GHS08  GHS08
Signal word 
Danger
Hazard statements 
Precautionary statements 
Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 1
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Buffers - From Bnech to Bulk
Protocols & Articles

Articles

TAE and TBE Running Buffers Recipe & Video

Tris acetate EDTA (TAE) and tris borate EDTA (TBE) are the two most common running buffers used in nucleic acid electrophoresis. As buffers, they have a fairly constant pH and are able to conduct ele...
Keywords: AGE, Buffers, Electrophoresis, Gel electrophoresis, Molecular biology, Peptide synthesis, Polymerase chain reaction, Precipitation, Pulsed field gel electrophoresis, Sequencing

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A buffer, as defined by Van Slyke [1], is "a substance which by its presence in solution increases the amount of acid or alkali that must be added to cause unit change in pH". Buffers are thus very i...
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Peer-Reviewed Papers
15

References

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