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T3934 Sigma

TRI Reagent®

LS, For processing fluid samples such as cell suspensions, CSF, and amniotic fluid.

Synonym: TRI Reagent® RNA Isolation Reagent

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Description

Application

TRI Reagent is an improved version of the single-step total RNA isolation reagent developed by Chomczynski. The RNA isolation method based on this reagent is widely used and proven for RNA applications. It is ideal for quick, economical, and efficient isolation of total RNA or the simultaneous isolation of RNA, DNA, and proteins from samples of human, animal, plant, yeast, bacterial, and viral origin.

The reagent has been used for isolating total RNA. The resulting RNA can be used for Northern blots, mRNA isolation, in vitro translation, RNase protection assay, cloning, and PCR.

Features and Benefits

• Easily scalable RNA isolation
• Works with many sources: human, plant, yeast, bacterial, or viral
• Better yields than traditional guanidine thiocyanate/cesium chloride methods

Packaging

100, 200 mL in glass bottle

General description

TRI Reagent LS is a quick and convenient reagent for use in the simultaneous isolation of RNA, DNA, and protein from liquid samples of human, animal, plant, yeast, bacterial, and viral origin.

A convenient single-step liquid phase separation results in the simultaneous isolation of RNA, DNA, and protein. This procedure is an adaptation of the singlestep method reported by Chomczynski and Sacchi for total RNA isolation, and permits fast and efficient processing of liquid samples. TRI Reagent LS performs well with large or small sample volumes, and many samples can be simultaneously extracted.

TRI Reagent LS is a mixture of guanidine thiocyanate and phenol in a monophase solution. When a biological sample is homogenized or lysed with it, and chloroform or 1-bromo-3-chloropropane is added, the mixture separates into 3 phases: an aqueous phase containing RNA, the interphase containing DNA, and an organic phase containing proteins. Each component can then be isolated after separating the phases. 0.75 ml of TRI Reagent LS processes 0.25 ml of a liquid sample such as amniotic fluid.

This is one of the most effective methods for isolating total RNA from fresh samples in only one hour. The procedure is very effective for isolating RNA molecules of all types from 0.1-15 kb in length. The resulting RNA is intact with little or no contaminating DNA and protein.

Legal Information

TRI Reagent is a registered trademark of Molecular Research Center, Inc.

Price and Availability


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Safety & Documentation

Safety Information

Signal word 
Danger
Supplemental Hazard Statements 
Contact with acids liberates very toxic gas.
RIDADR 
UN 2922 6.1(8) / PGIII
WGK Germany 
3
Flash Point(F) 
174.2 °F
Flash Point(C) 
79 °C
Protocols & Articles

Articles

Sample Purification & Quality Assessment - PCR Technologies Guide

The availability of simple methods for purification of DNA and RNA has greatly facilitated the analysis and characterization of the genome and gene expression. There is a demand to isolate DNA and RN...
Keywords: AGE, Absorption, Amplification, Bio-Analytical, Buffers, Capillary electrophoresis, Cell disruption, Centrifugation, Chromatin immunoprecipitation, DNA purification, Degradations, Detection methods, Detergents, Digestions, Electrophoresis, Extinction coefficient, Filtration, Gel electrophoresis, Gene expression, Indicators, Lab-on-a-chip, Molecular biology, Nucleic acid hybridization, Nucleic acid purification, Polymerase chain reaction, Polymerase chain reaction - quantitative, Precipitation, Purification, RNA purification, Separation, Spectroscopy, Transcription, Ultraviolet-Visible spectroscopy

Peer-Reviewed Papers
15

References

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