T4182 Sigma

Tyrphostin AG 1478


Synonym: N-(3-Chlorophenyl)-6,7-dimethoxy-4-quinazolinamine



Related Categories Bioactive Small Molecule Alphabetical Index, Bioactive Small Molecules, Biochemicals and Reagents, Cell Biology, Cell Signaling and Neuroscience,
assay   ≥98%
solubility   0.1 M HCl: soluble <0.4  mg/mL
  DMSO:methanol (1:1): soluble 10 mg/mL, clear, colorless
  ethanol: soluble 10 mg/mL
  methylene chloride: soluble 10  mg/mL
  0.1 M NaOH: insoluble
  2-hydroxypropyl-β-cyclodextrin: insoluble
  H2O: insoluble
storage temp.   2-8°C
Gene Information   human ... EGFR(1956)
mouse ... Egfr(13649)


Biochem/physiol Actions

Selective inhibitor of epidermal growth factor receptor protein

Studies in human glioma cell lines (U87MG) have shown that tyrphostin AG 1478 preferentially blocks truncated as opposed to the wild-type EGFR4.


1, 5 mg in ampule

Preparation Note

Tyrphostin AG 1478 dissolves in DMSO:MeOH (1:1) at 10 mg/ml to yield a clear, colorless solution. It is also soluble in methylene chloride (10 mg/ml), ethanol (10 mg/ml) and 0.1 N HCl (less than 0.4 mg/ml). However, it is insoluble in water, 2-hydroxypropyl-β-cyclodextrin, and 0.1 N NaOH.


Tyrphostin AG 1478 has been used as an EGF receptor (EGFR) inhibitor in SW1353 cultures2. AG 1478 is also known to inhibit exogenous TGFα-mediated c-Met phosphorylation in A431 cells3.

Features and Benefits

This compound is a featured product for Kinase Phosphatase Biology research. Click here to discover more featured Kinase Phosphatase Biology products. Learn more about bioactive small molecules for other areas of research at

This compound is also offered as part of Sigma′s Library of Pharmacologically Active Compounds (LOPAC®1280), a biologically annotated collection of high-quality, ready-to-screen compounds. Click here to learn more.

This compound is featured on the EGFR page of the Handbook of Receptor Classification and Signal Transduction. To browse other handbook pages, click here.

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Safety & Documentation

Safety Information

NONH for all modes of transport


Certificate of Analysis

Certificate of Origin

Protocols & Articles


Discover Bioactive Small Molecules for Cytokines & Growth Factors Research

Cytokines and growth factors (GF) are chemical messengers that mediate intracellular communication, thereby regulating cellular and nuclear functions. These chemical messengers bind cell surface rece...
Keywords: Biological processes, Cancer, Cell proliferation, Diseases, Growth factors, Ligands, Phosphorylations, Transcription, Transduction

Discover Bioactive Small Molecules for Kinase Phosphatase Biology

Phosphorylation is a ubiquitous cellular regulatory mechanism that mediates signal transduction pathways, which carry signals from the cell surface to the nucleus or cytoplasm, by altering the activi...
Keywords: Apoptosis, Bioactive small molecules, Cancer, Diseases, Inflammation, Metabolism, Neurodegenerative Diseases, Phosphorylations, Transduction

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