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T4330 Sigma-Aldrich

Turbonuclease from Serratia marcescens

recombinant, expressed in E. coli

Synonym: Endonuclease from Serratia marcescens

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Properties

Quality Level   200
recombinant   expressed in E. coli
form   liquid
concentration   ≥200,000 units/mL
storage temp.   −20°C

Description

General description

Turbonuclease provides a nuclease treatment by reducing viscosity and degrading RNA, genomic DNA, baculovirus DNA, and unencapsidated vector DNA.

Application

Turbonuclease has been used in a study to assess the TY3 gag3 spacer effect on intracellular condensation and uncoating.

Used for the removal of nucleic acid from protein samples.

Biochem/physiol Actions

Digests native or heat-denatured DNA and RNA.

Unit Definition

One unit will digest sonicated salmon sperm DNA to acid-soluble oligonucleotides equivalent to a ΔA260 of 1.0 in 30 min at pH 8.0 at 37 °C.

Physical form

Supplied as a solution in 50 mM Tris-HCl, pH 8.0 and 50 mM NaCl

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 2
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles
Peer-Reviewed Papers
15

References

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