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T5201 Sigma-Aldrich

Monoclonal Anti-β-Tubulin antibody produced in mouse

clone TUB 2.1, purified from hybridoma cell culture

Synonym: Monoclonal Anti-β-Tubulin

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies against Proteins/Bioactives/Markers/Receptors for Stem Cell Biology, Antibodies for Cell Biology, Antibodies to Cell and Organelle Proteins,
species reactivity   chicken, human, plant, frog, wheat, rabbit, bovine, hamster, moth, mouse, sea urchin, rat
application(s)   immunoelectrophoresis: suitable
  immunohistochemistry: suitable
  indirect immunofluorescence: 10-20 μg/mL using cultured chicken fibroblasts
  microarray: suitable
  western blot: 2-4 μg/mL using chicken fibroblasts cell extract
clone   TUB 2.1, monoclonal
concentration   ~2 mg/mL
antibody form   purified immunoglobulin
form   buffered aqueous solution
isotype   IgG1
mol wt   antigen mol wt 55 kDa
shipped in   dry ice
storage temp.   −20°C
biological source   mouse
antibody product type   primary antibodies
conjugate   unconjugated

Description

Immunogen

tubulin from rat brain.

General description

β-Tubulin belongs to the subfamily of tubulin, which is the major building block of microtubules. β-Tubulin has a molecular weight of 55kDa. β-Tubulin structure is characterized with core of two β-sheets enclosed by α-helices. It also contains an N-terminal domain with the guanine nucleotide-binding region, an intermediate domain with taxol-binding site, and a C-terminal domain that contains the binding surface for molecular motor proteins. Human β-tubulin consists of seven isoforms (βI (class I), βI (class II), βIII (class III), βIVa (class IVa), βIVb (class IVb), βV (class V), and βVI (class VI)).

Physical form

Solution in 0.01 M phosphate buffered saline containing 15 mM sodium azide.

Specificity

The antibody recognizes all five isoforms of β-tubulin (β1-β5). It reacts with the β-Lc and β-Sc fragments in the carboxy-terminal part of β−tubulin in immunoblotting. The antibody may be used to localize β−tubulin in cultured cells or tissue sections.

Application

Monoclonal Anti-β-Tubulin antibody produced in mouse has been used in western blotting.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

Mutation in the gene leads to various neuronal migration disorders such as lissencephaly, pachygyria and polymicrogyria malformations. B2702 peptide binds to β-tubulin and inhibits NK cell cytotoxicity and it influences microtubule polymerization, which damages cytoskeleton organization and chaperone-like activity of tubulin. β-1 tubulin also known as class VI or TUBB1, plays a vital role in platelet production, and is considered to be a potential biomarker candidate for immune thrombocytopenia (ITP).

Safety & Documentation

Safety Information

Personal Protective Equipment  Eyeshields, Gloves, half-mask respirator (US), multi-purpose combination respirator cartridge (US)
RIDADR  NONH for all modes of transport
WGK Germany  3
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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