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T5398 Sigma-Aldrich

Transglutaminase from guinea pig liver

lyophilized powder, ≥1.5 units/mg protein

Synonym: Protein-glutamine:amine γ-glutamyltransferase, Protein-Glutamine-γ-Glutamyltransferase

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Properties

Related Categories 2.3.x.x Acyltransferases, 2.x.x.x Transferases, Application Index, Biochemicals and Reagents, Cell Biology,
form   lyophilized powder
composition   Protein, ≥80% modified Warburg-Christian
solubility   H2O: soluble 1.0 mg/mL, clear
Featured Industry   Diagnostic Assay Manufacturing
shipped in   dry ice
storage temp.   −20°C

Description

Application

This product from Sigma has been used to demonstrate that tissue transglutaminase (tTG) selectively deamidates gluten peptides, which results in strongly enhanced T cell-stimulatory activity. It has also been used to assess immune responses to A-gliadin peptides. Furthermore, it has been used to demonstrate that tTG selectively modifies gliadin peptides that are recognized by gut-derived T cells in celiac disease.

Transglutaminase has been used in a study to improve quantifiable assays to fully characterize the role of transglutaminase in diseases such as Huntington′s disease and Alzheimer′s disease.Transglutaminase has also been used in a study to develop a nonradioactive dot blot assay for transglutaminase activity.

Biochem/physiol Actions

Transglutaminase from guinea pig liver consists of a single polypeptide chain of 691 amino acid residues. It has six potential glycosylation sites (Asn-X-Ser or Asn-X-Thr), but it is not glycosylated. The molecular mass is approximately 76.6 kDa. It is calcium dependent and has several calcium binding sites. The enzyme is inhibited by iodoacetamide and N-ethylmaleimide in the presence of calcium. It catalyzes the incorporation of small molecular weight amines into γ-glutamine sites of proteins. In the absence of small molecular weight amines, it catalyzes the cross linking of proteins that results in the formation of γ-glutamyl-ε-lysine side chain peptides. Liver transglutaminase is a nonzymogenic enzyme.

Unit Definition

One unit will catalyze the formation of 1.0 μmole of hydroxamate per min from Nα-Z-Gln-Gly and hydroxylamine at pH 6.0 at 37 °C. (L-Glutamic acid γ-monohydroxamate is the standard.)

Physical form

Lyophilized powder containing Tris and dithioerythritol

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
1
RTECS 
MA2370000

Documents

Certificate of Analysis

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Certificate of Origin

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Protocols & Articles

Related Content

Enzymes & Proteins

Application Index | Enzyme Index | Substrate Index | Inhibitor Index | Cofactor Index | Lectin Index
Keywords: Cell signaling, Diagnostic, Drug discovery, Metabolomics, Molecular biology

Peer-Reviewed Papers
15

References

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