• USA Home
  • T5425 - Transforming Growth Factor-β3 human

EMAIL THIS PAGE TO A FRIEND
T5425 Sigma-Aldrich

Transforming Growth Factor-β3 human

TGF-β3, recombinant, expressed in baculovirus infected Sf21 cells, powder, suitable for cell culture

Synonym: TGF-β3, hTGF-β3

Purchase

Properties

Related Categories Cancer Research, Cell Biology, Cell Culture, Cell Signaling and Neuroscience, Cytokines & Growth Factors,
biological source   human
recombinant   expressed in baculovirus infected Sf21 cells
assay   ≥97% (SDS-PAGE)
form   powder
potency   0.01-0.09 ng/mL ED50/EC50
quality   endotoxin tested
mol wt   predicted mol wt ~25 kDa
packaging   pkg of 2 μg
storage condition   avoid repeated freeze/thaw cycles
impurities   <0.01 EU/μg
suitability   suitable for cell culture
UniProt accession no.   P10600
storage temp.   −20°C
Gene Information   human ... TGFB3(7043)

Description

General description

The TGFβ3 (transforming Growth Factor-β3) gene is mapped to human chromosome 14q24.3.

Application

Transforming Growth Factor-β3 human has been used to study the role of TGF-β3 in healing tendon-to-bone insertion and in chondrogenic differentiation.

Biochem/physiol Actions

TGF-β (transforming growth factor-β3) isoforms are significantly involved in inducing inflammatory responses. It is associated with vascular diseases, wound healing and ameliorating effect in venous leg ulcer. TGF-β3 has an antagonistic effect on TGF-β1 and reduces the degree of collagen type I deposition and fibroblast differentiation. As a result TGF-β3 might lead to ulcer chronicity due to hindered myofibroblasts formation. TGF-β is a key player in tumor formation and progression. Increased TGF-β3 expression is observed in cancer and adipose tissue fibrosis. TGF-β3 is crucial in tissue regeneration and scarless tissue repair.

TGF-β3 is less prevalent in natural expression than either TGF-β1 or TGF-β2, but it is the most abundant mRNA expressed in chick embryos. It is also expressed in human umbilical cord, in a variety of mesenchymal cells of human and rodent origin, and in several human carcinoma cells.

Physical form

Lyophilized from a 0.2 μm filtered solution in 35% acetonitrile and 0.1% trifluoroacetic acid containing 50 μg bovine serum albumin per 1 μg of cytokine.

Analysis Note

The biological activity is measured by its ability to inhibit the IL-4-dependent proliferation of mouse HT-2 cells.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

???PDP_SDS_LOT_NUMBER_ERROR_MSG???

Certificate of Origin

???PDP_SDS_LOT_NUMBER_ERROR_MSG???

Request a Serum Sample

Cell Culture Guide
Protocols & Articles

Related Content

Cell Culture Troubleshooting Guide

Cell culture has become one of the most fundamental techniques for modeling biological systems, and is of increasing importance in the biotechnology and pharmaceutical sectors as well as an essential...
Keywords: Antibiotics, Apoptosis, Cell attachment, Cell culture, Cryopreservation, Filtration, Growth factors, Pharmaceutical, Phase transitions

Peer-Reviewed Papers
15

References

Related Products

related product

Product #

Description

Add to Cart

92210 Timestrip Plus 0 °C
06693 Timestrip Plus -20 °C

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?