• USA Home
  • T5516 - 3,3′,5-Triiodo-L-thyronine sodium salt

T5516 Sigma-Aldrich

3,3′,5-Triiodo-L-thyronine sodium salt

γ-irradiated, powder, suitable for, suitable for cell culture

Synonym: Liothyronine, O-(4-Hydroxy-3-iodophenyl)-3,5-diiodo-L-tyrosine sodium salt, T3

  • CAS Number 55-06-1

  • Empirical Formula (Hill Notation) C15H11I3NNaO4

  • Molecular Weight 672.96

  •  Beilstein/REAXYS Number 8179867

  •  EC Number 200-223-5

  •  MDL number MFCD00002594

  •  PubChem Substance ID 24900290

  •  NACRES NA.77



Related Categories Cell Biology, Cell Culture, Cell Signaling and Neuroscience, Cytokines, Growth Factors and Hormones, Hormones,
Quality Level   200
biological source   synthetic (organic)
sterility   γ-irradiated
assay   ≥90% (HPLC)
form   powder
application(s)   cell culture | mammalian: suitable
suitability   suitable for
shipped in   ambient
storage temp.   −20°C
SMILES string   [Na+].N[C@@H](Cc1cc(I)c(Oc2ccc(O)c(I)c2)c(I)c1)C([O-])=O
InChI   1S/C15H12I3NO4.Na/c16-9-6-8(1-2-13(9)20)23-14-10(17)3-7(4-11(14)18)5-12(19)15(21)22;/h1-4,6,12,20H,5,19H2,(H,21,22);/q;+1/p-1/t12-;/m0./s1
Gene Information   human ... THRA(7067), THRB(7068)
rat ... Thra(81812), Thrb(24831)


General description

3,3′,5′-Triiodo-L-thyronine (T3) is an endocrine hormone produced by the thyroid gland and is regarded as an active thyroid hormone at cellular level. Thyroxine (T4) serves as a precursor for T3 and is generated in extrathyroidal tissues.


3,3′,5-Triiodo-L-thyronine sodium salt has been used:
• as a supplement in colon organ culture media
• to stimulate HuH-7 cells for analysing the hormonal influence on MBL synthesis
• to study its effect on human growth hormone (GH) versus mouse GH expression in the presence of ′′intact′′ GH genes and in a common pituitary cell context

Biochem/physiol Actions

3,3′,5′-Triiodo-L-thyronine (T3) is a thyroid hormone that increases rates of proteins synthesis, stimulates the breakdown of cholesterol, and effects embryonic development. In cell culture, T3 regulates cell differentiation and protein expression. The interaction of T3 with nonhistone proteins in the chromatin initiates the thyroid hormone activity. Thyroid hormones regulate the differentiation and development of tissues and affect a number of metabolic processes. The level of circulating T3 hormone is reduced in hypothyroidism.

Physical form

powder-0 °C; stock-frozen in working aliquots, avoid repeated freeze/thaw, solution stable 2-8 °C, 30 days

Preparation Note

Package size based on 3,3′,5-Triiodo-L-thyronine sodium salt content; vial may also contain inert salts, such as sodium chloride


To prepare 20 μg/ml stock solution: add 1 ml 1 N NaOH; gently swirl to dissolve; add 49 ml sterile medium.

Safety & Documentation

Safety Information

Personal Protective Equipment 
NONH for all modes of transport
WGK Germany 
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable


Certificate of Analysis (COA)

Please Enter a Lot Number

Certificate of Origin (COO)

Please Enter a Lot Number
Protocols & Articles


Derivation of Functional Oligodendrocyte Progenitor Cells (OPCs) from Human Neural Stem Cell Lines

Introduction Methods    Human Neural Stem Cell Culture    Oligodendrocyte Differentiation and Maturation    Oligodendrocyte Progenitor Cell Characterization    In Vitro Myelination Assay Results Conc...
Christine Chen, Michael Moeller, Anna Abai, Nick Asbrock and Vi Chu1
MilliporeSigma, Bioscience Division, Temecula, CA, USA
Keywords: Adhesion, Atomic absorption spectroscopy, Cell culture, Cell proliferation, Central Nervous System, Clinical, Culture media, Diseases, Gene expression, Growth factors, Hormones, Neurodegenerative Diseases, Pharmaceutical, Phase transitions, RNA immunoprecipitation, Transfection

Related Content

Cell Culture Troubleshooting Guide

Cell culture has become one of the most fundamental techniques for modeling biological systems, and is of increasing importance in the biotechnology and pharmaceutical sectors as well as an essential...
Keywords: Antibiotics, Apoptosis, Cell attachment, Cell culture, Cryopreservation, Filtration, Growth factors, Pharmaceutical, Phase transitions

Peer-Reviewed Papers


Related Products

related product

Product #


Add to Cart

92210 Timestrip Plus 0 °C
06693 Timestrip Plus -20 °C

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?