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  • T5567 - Monoclonal Anti-TCF-1 (T-Cell Factor-1) antibody produced in mouse

T5567 Sigma

Monoclonal Anti-TCF-1 (T-Cell Factor-1) antibody produced in mouse

~1 mg/mL, clone 7H3, purified immunoglobulin, buffered aqueous glycerol solution



Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Gene Regulation, Antibodies for Hematopoietic Stem Cells,
species reactivity   human
application(s)   immunohistochemistry (frozen sections): suitable
  immunoprecipitation (IP): suitable
  western blot: 0.5-2 μg/mL using whole extracts of Jurkat cells
clone   7H3, monoclonal
concentration   ~1 mg/mL
antibody form   purified immunoglobulin
form   buffered aqueous glycerol solution
isotype   IgG
mol wt   antigen mol wt 26-48 kDa (32, 38, 42)
shipped in   wet ice
storage temp.   −20°C
Gene Information   human ... HNF1A(6927)
biological source   mouse
conjugate   unconjugated



MBP fusion protein corresponding to amino acids 109-269 of human TCF-1A splice variant.

Physical form

Solution in 0.1 M tris-glycine, pH 7.4, containing 0.15 M sodium chloride, 30% glycerol and 0.08% sodium azide.

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Certificate of Analysis

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WGK Germany  1
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Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy


Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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