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  • T5817 - Monoclonal Anti-TCF-4 (T-Cell Factor-4) antibody produced in mouse

T5817 Sigma-Aldrich

Monoclonal Anti-TCF-4 (T-Cell Factor-4) antibody produced in mouse

~1 mg/mL, clone 6H5-3, purified immunoglobulin, buffered aqueous glycerol solution



Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Epigenetics and Nuclear Signaling, Antibodies for Gene Regulation,
conjugate   unconjugated
clone   6H5-3, monoclonal
biological source   mouse
application(s)   immunohistochemistry: suitable using 4% formaldehyde, paraffin-embedded human and mouse tissue
  immunoprecipitation (IP): suitable
  western blot: 1-4 μg/mL using whole extracts of Cos-1 cells transfected with human TCF-4
species reactivity   mouse, human
mol wt   antigen 66 kDa
form   buffered aqueous glycerol solution
shipped in   wet ice
storage temp.   −20°C
antibody form   purified immunoglobulin
isotype   IgG2a
Quality Level   200
antibody product type   primary antibodies
concentration   ~1 mg/mL
UniProt accession no.   Q9NQB0
Gene Information   human ... TCF7L2(6934)
mouse ... Tcf7l2(21416)


General description

Transcription factor 4 or T-Cell Factor-4 (TCF-4), also called as transcription factor 7 like 2 (TCF7L2), is mapped to human chromosome 18q21.1. The gene codes for a basic helix-loop-helix DNA binding protein, which belongs to TCF family and is implicated in transcriptional regulation. It is expressed predominantly in nervous system. TCF4 protein has a transcriptional activation domain 2 (AD2), a transcriptional activation domain (AD1) and a nuclear localization signal (NLS) in the N-terminal end and a C-terminal basic helix-loop-helix (bHLH) domain, involved in dimerization and deoxyribonucleic acid (DNA) binding.


His-tagged fusion protein corresponding to amino acids 31-331 of human TCF-4


May be used for gel-shift assays

Physical form

Solution in 0.7 M tris-glycine, pH 7.4, containing 0.105 M sodium chloride, 30% glycerol and 0.035% sodium azide.


Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

Transcription factor 4 (TCF-4) interacts functionally with β-catenin to mediate Wnt signaling pathway and transactivates downstream target genes implicated in cancer development. TCF-4 plays a vital role in development of the nervous system and vertebrate development. Aberrations or mutation of the gene leads to Pitt-Hopkins syndrome (PTHS), a rare developmental disorder characterized by severe motor and mental retardation, typical facial features and breathing abnormalities.

Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable


Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles


Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy


Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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