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  • T5826 - Anti-TSG101 (N-terminal) antibody produced in rabbit

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T5826 Sigma-Aldrich

Anti-TSG101 (N-terminal) antibody produced in rabbit

~1 mg/mL, affinity isolated antibody, buffered aqueous solution

Synonym: Anti-Tumor Susceptibility Gene 101

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Cell Cycle, Antibodies for Epigenetics and Nuclear Signaling,
conjugate   unconjugated
clone   polyclonal
biological source   rabbit
application(s)   microarray: suitable
  western blot: 1-2 μg/mL using cell lysates of rat fibroblast Rat-1, mouse fibroblast NIH3T3, and human A431 cells, and rat brain extract (S1 cytosolic fraction)
species reactivity   rat, human, mouse
mol wt   antigen 46 kDa
form   buffered aqueous solution
shipped in   dry ice
storage temp.   −20°C
antibody form   affinity isolated antibody
antibody product type   primary antibodies
concentration   ~1 mg/mL
UniProt accession no.   Q99816
Gene Information   human ... TSG101(7251)
mouse ... Tsg101(22088)
rat ... Tsg101(292925)

Description

General description

Tumor susceptibility gene 101 protein (TSG101) is mapped to human chromosome 11p15.1. TSG101 has ubiquitin E2 variant domain.

Immunogen

synthetic peptide corresponding amino acids 2-20 located at the N-terminus of human TSG101, conjugated to KLH. This sequence is identical in rat and mouse TSG101.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

Tumor susceptibility gene 101 protein (TSG101) is a component of endosomal sorting complex required for transport (ESCRT) machinery. TSG101 interacts with arrestin domain-containing protein 1 and mediates its budding through plasma membrane. It binds to ubiquitinylated proteins and conveys them for degradation by lysosomes. TSG101 is also essential for the budding human immunodeficiency virus (HIV) infected cells. TSG101 deficiency is associated with genome instability and is considered as a risk factor for tumor initiation.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

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Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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T5951 Anti-TSG101 (C-terminal) antibody produced in rabbit, ~1.5 mg/mL, affinity isolated antibody, buffered aqueous solution
T5701 Anti-TSG101 antibody produced in rabbit, ~2 mg/mL, affinity isolated antibody, buffered aqueous solution

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