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T5941 Sigma-Aldrich

Trizma® hydrochloride

BioPerformance Certified, cell culture tested, ≥99.0% (titration)

Synonym: TRIS HCl, TRIS hydrochloride, Tris(hydroxymethyl)aminomethane hydrochloride

  • CAS Number 1185-53-1

  • Linear Formula NH2C(CH2OH)3 · HCl

  • Molecular Weight 157.60

  •  Beilstein Registry Number 3675235

  •  EC Number 214-684-5

  •  MDL number MFCD00012590

  •  PubChem Substance ID 24900332

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Properties

Related Categories Apoptosis and Cell Cycle, BioPerformance Certified Buffers, Biochemicals and Reagents, Biological Buffers, Buffer / Buffer Salts,
Quality Level   ELITE
grade   BioPerformance Certified
InChI Key   QKNYBSVHEMOAJP-UHFFFAOYSA-N
assay   ≥99.0% (titration)
impurities   DNAse, Exonuclease; NICKase, Endonuclease; RNAse and Protease, none detected
  endotoxin and total aerobic microbial count, tested
  ≤0.5% water (Karl Fischer)
useful pH range   7.0 - 9.0
pKa (25 °C)   8.1
mp   150-152 °C
solubility   H2O: 667 mg/mL
absorption   A40%/290 ≤0.05
suitability   cell culture tested
  suitable for electrophoresis
Featured Industry   Diagnostic Assay Manufacturing

Description

Other Notes

Easily compare specifications for Trizma HCl products with the Trizma HCl specification table.

Packaging

1 kg in poly bottle

100, 500 g in poly bottle

5 kg in poly drum

Application

The pH values of all buffers are temperature and concentration dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.
For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.

Legal Information

Trizma is a registered trademark of Sigma-Aldrich Co. LLC

Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
1

Protein Stabilizer
Protocols & Articles

Protocols

Arabidopsis mesophyll protoplasts: a versatile cell system for transient gene expression analysis

The transient gene expression system using Arabidopsis mesophyll protoplasts has proven an important and versatile tool for conducting cell-based experiments using molecular, cellular, biochemical, g...
Keywords: Gene expression, Genetic, Genetics, Molecular biology, Transfection

Investigation of higher-order RNA G-quadruplex structures in vitro and in living cells by 19F NMR spectroscopy

a Division of Chemistry, Department of Medical Sciences, Faculty of Medicine, University of Miyazaki
Keywords: Cancer, Capillary electrophoresis, Cell disruption, Chromatography, Click chemistry, Electron spin resonance, Gene expression, High performance liquid chromatography, Indicators, Industries, Ligands, Liquid chromatography mass spectrometry, Mass spectrometry, Melting, Methylations, Nuclear magnetic resonance spectroscopy, Nucleic acid annealing, Protein assay, Purification, RNA purification, Separation, Solvents, Spectroscopy, Thin layer chromatography, Transcription

Related Content

BioUltra Biological Buffers

A buffer, as defined by Van Slyke [1], is "a substance which by its presence in solution increases the amount of acid or alkali that must be added to cause unit change in pH". Buffers are thus very i...
Keywords: Absorption, Adsorption, Biochemistry, Biological Buffers, Biological processes, Buffers, PAGE

Peer-Reviewed Papers
15

References

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