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T6066 Sigma-Aldrich

Trizma® base

BioPerformance Certified, meets EP, USP testing specifications, cell culture tested, ≥99.9% (titration)

Synonym: 2-Amino-2-(hydroxymethyl)-1,3-propanediol, THAM, Tris base, Tris(hydroxymethyl)aminomethane, Trometamol

  • CAS Number 77-86-1

  • Linear Formula NH2C(CH2OH)3

  • Molecular Weight 121.14

  •  Beilstein Registry Number 741883

  •  EC Number 201-064-4

  •  MDL number MFCD00004679

  •  PubChem Substance ID 24900345

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Properties

Related Categories BioPerformance Certified Buffers, Biochemicals and Reagents, Biological Buffers, Buffer / Buffer Salts, Buffered Salt Solutions,
Quality Level   ELITE
grade   BioPerformance Certified
description   aminopeptidase substrate
InChI Key   LENZDBCJOHFCAS-UHFFFAOYSA-N
assay   ≥99.9% (titration)
form   crystalline powder
impurities   DNase, RNase, protease, none detected
  Endotoxin and Total Count Aerobic, Yeast, plus Mold, tested
  ≤0.2% water (Karl Fischer)
loss   ≤0.5% (loss on drying, 110 °C)
pH   10.0-11.5
useful pH range   7 - 9
pKa (25 °C)   8.1
bp   219-220 °C/10 mmHg(lit.)
mp   167-172 °C (lit.)
solubility   water: soluble (666 mg/ml, yielding a clear colorless solution)
absorption   A1M/260 ≤0.05
  A1M/280 ≤0.06
  A40%/290 ≤0.05
suitability   cell culture tested
  meets EP, USP testing specifications
Featured Industry   Diagnostic Assay Manufacturing

Description

Other Notes

Easily compare specifications for Trizma products with the Trizma specification table.

The pH values of all buffers are temperature- and concentration-dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.
For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.

Packaging

1 kg in poly bottle

100, 500 g in poly bottle

5 kg in poly drum

Application

Trizma has been used:
• As a component in the culture of human embryonic stem (hES) cells on human and mouse feeder cells
• As a component of pre-hybridization buffer in in situ hybridization
• To suspend dried protein before SDS-PAGE protein characterization
• As a component of lysis buffer to wash and lyse cells before immunoprecipitation
• In the extraction and enrichment of membrane proteins for separation by two-dimensional polyacrylamide gel electrophoresis
• In studies of double stranded complexes of peptide nucleic acids (PNA) and their complementary DNA sequences, by use of anion exchange HPLC
• In capillary electrochromatography and UV analysis of tocopherols and tocotrienols

General description

Tris is an established basimetric standard and buffer used in biochemistry and molecular biology. It may be used by itself as a buffer or as a component of mixed buffer formulations, such as Tris-EDTA (TE) buffer, Tris-acetate-EDTA (TAE) buffer, Tris-borate-EDTA (TBE) buffer, etc. It is pure, essentially stable, relatively non-hygroscopic and has a high equivalent weight.

Legal Information

Trizma is a registered trademark of Sigma-Aldrich Co. LLC

Protocols & Articles

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Peer-Reviewed Papers
15

References

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