T6146 Sigma-Aldrich

Trypan Blue

powder, BioReagent, suitable for cell culture

Synonym: Direct blue 14

  • CAS Number 72-57-1

  • Linear Formula C34H24N6O14S4Na4

  • Molecular Weight 960.81

  •  Colour Index Number 23850

  •  Beilstein/REAXYS Number 4360496

  •  EC Number 200-786-7

  •  MDL number MFCD00003969

  •  PubChem Substance ID 57654686

  •  NACRES NA.75



Related Categories Apoptosis and Cell Cycle, Assays and Reagents for Measuring Cytotoxicity, Proliferation and Viability, Base Ingredients for Media, Cell Analysis, Cell Biology,
Quality Level   200
product line   BioReagent
form   powder
composition   Dye content, ~40%
packaging   glass bottle of 100 g
  glass bottle of 25 g
  glass bottle of 5 g
storage condition   dry at room temperature
application(s)   cell analysis: suitable
  cell culture | mammalian: suitable
  tissue processing: suitable
mp   >300 °C (lit.)
solubility   H2O: 10 mg/mL
SMILES string   [Na+].[Na+].[Na+].[Na+].Cc1cc(ccc1N=Nc2c(O)c3c(N)cc(cc3cc2S([O-])(=O)=O)S([O-])(=O)=O)-c4ccc(N=Nc5c(O)c6c(N)cc(cc6cc5S([O-])(=O)=O)S([O-])(=O)=O)c(C)c4
InChI   1S/C34H28N6O14S4.4Na/c1-15-7-17(3-5-25(15)37-39-31-27(57(49,50)51)11-19-9-21(55(43,44)45)13-23(35)29(19)33(31)41)18-4-6-26(16(2)8-18)38-40-32-28(58(52,53)54)12-20-10-22(56(46,47)48)14-24(36)30(20)34(32)42;;;;/h3-14,41-42H,35-36H2,1-2H3,(H,43,44,45)(H,46,47,48)(H,49,50,51)(H,52,53,54);;;;/q;4*+1/p-4
Gene Information   human ... PTPN1(5770), PTPRF(5792)


General description

The trypan blue dye is an azo dye, derived from toluidine. It is soluble in water and has a molecular weight of 961 g/mol.


Trypan blue has been used:
• in cell viability assay
• to determine the number of living and necrotic cells by staining with 0.2% trypan blue solution.
• to stain plant tissue in order to to visualize cell death


5, 25, 100 g in glass bottle

Biochem/physiol Actions

The trypan blue dye is useful to differentiate the dead and live cells based on the cell′s ability to take up or exclude the dye. A viable cell is assumed to possess an intact cell membrane and hence excludes this dye and the cytoplasm appears clear. While on the other hand non-viable cells shows a blue cytoplasm.

Safety & Documentation

Safety Information

GHS08  GHS08
Signal word 
Hazard statements 
UN 3077 9 / PGIII
WGK Germany 


Certificate of Analysis (COA)

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Certificate of Origin (COO)

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