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T6451 Sigma-Aldrich

Anti-Twist1 antibody produced in rabbit

~1 mg/mL, affinity isolated antibody, buffered aqueous solution

Synonym: Anti-Twist homolog 1 (acrocephalosyndactyly 3, Saethre-Chotzen syndrome), Anti-Twist related protein 1 (H-twist)

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies against Proteins/Bioactives/Markers/Receptors for Stem Cell Biology, Antibodies for Cell Biology, Antibodies for Epigenetics and Nuclear Signaling,
conjugate   unconjugated
clone   polyclonal
biological source   rabbit
application(s)   indirect immunofluorescence: 1-2 μg/mL using human osteosarcoma MG-63 cells
  western blot (chemiluminescent): 0.5-1 μg/mL using human 293T cells expressing recombinant human Twist1
species reactivity   rat, mouse, human
mol wt   antigen ~26 kDa
form   buffered aqueous solution
shipped in   dry ice
storage temp.   −20°C
antibody form   affinity isolated antibody
antibody product type   primary antibodies
concentration   ~1 mg/mL
UniProt accession no.   Q15672
Gene Information   human ... TWIST1(7291)
mouse ... Twist1(22160)
rat ... Twist1(85489)

Description

Specificity

Anti-Twist1 antibody recognizes human Twist1. Detection of the Twist1 band by immunoblotting is specifically inhibited with the immunizing peptide.

Immunogen

synthetic peptide corresponding to amino acids 12-27 of human Twist1 with a C-terminal added cysteine, conjugated to KLH. The corresponding peptide sequence is identical in both rat and mouse.

Application

Anti-Twist1 antibody is suitable for use in detecting Twist1 via in immunochemical applications such as immunoblotting (~26 kDa) and immunofluorescence.
Immunoblotting: a working antibody concentration of 0.5-1 mg/mL is recommended using extracts of human 293T cells expressing recombinant human Twist1 and a chemiluminescent detection reagent.

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Chromatin immunoprecipitation (1 paper)

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

Twist1 is a regulator of embryonic morphogenesis and plays an essential role in metastasis by promoting an epithelial-mesenchymal transition (EMT). During an EMT, Twist induces the expression of mesenchymal markers, such as fibronectin and N-cadherin, and represses E-cadherin expression inhibiting cell-cell adhesion and therefore inducing infiltrative tumor growth in various human carcinomas. Twist1 is required in head mesenchyme for cranial neural tube morphogenesis in mice. Twist1 is involved in osteoblast differentiation and maturation. Twist over-expression in breast cancer cells can induce angiogenesis and correlates with chromosomal instability.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

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Certificate of Origin

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Milli-Q® Water Purification Solutions
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

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Peer-Reviewed Papers
15

References

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WH0007291M1 Monoclonal Anti-TWIST1 antibody produced in mouse, clone 3E11, purified immunoglobulin, buffered aqueous solution
AV37997 Anti-TWIST1 antibody produced in rabbit, IgG fraction of antiserum

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