• USA Home
  • T6763 - Trypsin Acetylated from bovine pancreas

EMAIL THIS PAGE TO A FRIEND
T6763 Sigma-Aldrich

Trypsin Acetylated from bovine pancreas

Type V-S, ≥8,500 BAEE units/mg protein (biuret)

Synonym: Acetyltrypsin

Purchase

Description

General description

The trypsin molecule has two domains: one is related to the enzyme active site and the tryptophan residues; the other is related to the 8-anilinonaphthalene-1-sulfonate binding.

Application

Trypsin has been used in a study to assess the ontogeny of the endocrine pancreas in the fetal/newborn baboon. Trypsin digestion and hydrogen/deuterium exchange support the transition between inward- and outward-facing conformations during the catalytic cycle of the bacterial multidrug ATP-binding cassette transporter.

Packaging

1 g in glass bottle

100, 250 mg in glass bottle

Biochem/physiol Actions

Lys-acetylated trypsin is less susceptible to autolysis than the native form.

Unit Definition

One BAEE unit will produce a ΔA253 of 0.001 per min at pH 7.6 at 25° C using BAEE as substrate. Reaction volume = 3.2 mL (1 cm light path).

Preparation Note

Prepared by the method of Labouesse, J. and Gervais, M., Eur. J. Biochem., 2, 215 (1967).

Other Notes

View more information on trypsin at www.sigma-aldrich.com/enzymeexplorer.

Safety & Documentation

Safety Information

Symbol 
Signal word 
Danger
Hazard statements 
Precautionary statements 
Target organs 
Respiratory system
Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3

Documents

Certificate of Analysis (COA)

Please Enter a Lot Number

Certificate of Origin (COO)

Please Enter a Lot Number
Protocols & Articles

Related Content

Enzymes & Proteins

Committed to developing and providing breakthrough problem-solving reagents and solutions, explore our comprehensive offering of enzymes and protein reagents and kits with the confidence that you wil...
Keywords: Cell signaling, Diagnostic, Enzyme-linked immunosorbent assay, Immunohistochemistry, Metabolism, Molecular biology, Purification, Western blot

Peer-Reviewed Papers
15

References

Related Products

inhibitor

Product #

Description

Add to Cart

B6506 Benzamidine hydrochloride hydrate, ≥99%
T9003 Trypsin inhibitor from Glycine max (soybean), lyophilized powder
M6159 α2-Macroglobulin from human plasma, BioUltra, ≥98% (SDS-PAGE)
D7910 3,4-Dichloroisocoumarin, serine protease inhibitor
D0879 Diisopropylfluorophosphate
L2023 Leupeptin trifluoroacetate salt, ≥90% (HPLC), microbial
A6191 Antipain dihydrochloride from microbial source, protease inhibitor
T9777 Trypsin-chymotrypsin inhibitor from Glycine max (soybean), lyophilized powder
A1153 Aprotinin from bovine lung, lyophilized powder, 3-8 TIU/mg solid
P7626 Phenylmethanesulfonyl fluoride, ≥98.5% (GC)
A9024 α1-Antitrypsin from human plasma, salt-free, lyophilized powder
A6150 α1-Antitrypsin from human plasma, salt-free, lyophilized powder
T2011 Trypsin inhibitor from chicken egg white, Type III-O (free of ovoinhibitor)

substrate

Product #

Description

Add to Cart

B3279 Nα-Benzoyl-L-arginine 4-nitroanilide hydrochloride, >98% (TLC)
H6268 Hide–Remazol Brilliant Blue R, protease substrate
B4500 Nα-Benzoyl-L-arginine ethyl ester hydrochloride, trypsin substrate
B4750 Nα-Benzoyl-DL-arginine β-naphthylamide hydrochloride, trypsin substrate
B7632 N-Benzoyl-Phe-Val-Arg-p-nitroanilide hydrochloride, protease substrate
B4153 Boc-Gln-Ala-Arg-7-amido-4-methylcoumarin hydrochloride, ~95%

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?