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T6791 Sigma-Aldrich

Trizma® base

BioXtra, pH 10.5-12.0 (1 M in H2O), ≥99.9% (titration)

Synonym: 2-Amino-2-(hydroxymethyl)-1,3-propanediol, THAM, Tris base, Tris(hydroxymethyl)aminomethane, Trometamol

  • CAS Number 77-86-1

  • Linear Formula NH2C(CH2OH)3

  • Molecular Weight 121.14

  •  Beilstein/REAXYS Number 741883

  •  EC Number 201-064-4

  •  MDL number MFCD00004679

  •  PubChem Substance ID 24900405

  •  NACRES NA.25

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Properties

Related Categories BioXtra Buffers, Biochemicals and Reagents, Biological Buffers, Buffers A to Z, Good’s Buffers More...
Quality Level   400
description   aminopeptidase substrate
product line   BioXtra
assay   ≥99.9% (titration)
form   crystalline
impurities   ≤0.005% Insoluble matter
ign. residue (900°C)   ≤0.01% (as SO4)
loss   ≤0.5% loss on drying, 110°C
pH   10.5-12.0 (1 M in H2O)
useful pH range   7 - 9
pKa (25 °C)   8.1
bp   219-220 °C/10 mmHg (lit.)
mp   167-172 °C (lit.)
solubility   H2O: 1 M, colorless
anion traces   chloride (Cl-): ≤0.005%
  sulfate (SO42-): ≤0.005%
cation traces   Al: ≤0.0005%
  As: ≤0.0001%
  Ba: ≤0.0005%
  Bi: ≤0.0005%
  Ca: ≤0.001%
  Cd: ≤0.0005%
  Co: ≤0.0005%
  Cr: ≤0.0005%
  Cu: ≤0.0005%
  Fe: ≤0.0005%
  K: ≤0.005%
  Li: ≤0.0005%
  Mg: ≤0.0005%
  Mn: ≤0.0005%
  Mo: ≤0.0005%
  Na: ≤0.005%
  Ni: ≤0.0005%
  Pb: ≤0.0005%
  Sr: ≤0.0005%
  Zn: ≤0.0005%
absorption   ≤0.020 at 280 in H2O at 1 M
  ≤0.025 at 260 in H2O at 1 M
Featured Industry   Diagnostic Assay Manufacturing
SMILES string   NC(CO)(CO)CO
InChI   1S/C4H11NO3/c5-4(1-6,2-7)3-8/h6-8H,1-3,5H2
InChI key   LENZDBCJOHFCAS-UHFFFAOYSA-N

Description

Packaging

1 kg in poly bottle

100, 500 g in poly bottle

Other Notes

Easily compare specifications for Trizma products with the Trizma specification table.

The pH values of all buffers are temperature- and concentration-dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.
For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.

Legal Information

Trizma is a registered trademark of Sigma-Aldrich Co. LLC

Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
1
RTECS 
TY2900000

pHast Pack
Protocols & Articles

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Peer-Reviewed Papers
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References

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