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T6793 Sigma-Aldrich

Monoclonal Anti-Tubulin, Acetylated antibody produced in mouse

clone 6-11B-1, ascites fluid

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies to Cell and Organelle Proteins, Antibodies to Cytoskeleton,
conjugate   unconjugated
clone   6-11B-1, monoclonal
biological source   mouse
application(s)   dot blot: suitable
  electron microscopy: suitable
  immunocytochemistry: suitable
  indirect ELISA: suitable
  radioimmunoassay: suitable
  western blot: 1:2,000 using a bovine or rat brain preparation
species reactivity   mouse, rat, chicken, protista, bovine, invertebrates, human, monkey, pig, frog, plant, hamster
shipped in   dry ice
storage temp.   −20°C
antibody form   ascites fluid
isotype   IgG2b
Quality Level   200
antibody product type   primary antibodies
contains   15 mM sodium azide
Featured Industry   Research Pathology
UniProt accession no.   P68366
Gene Information   human ... TUBA4A(7277)
mouse ... Tuba1a(22142)
rat ... Tuba1a(64158)

Description

General description

α-tubulin, also called as tubulin α 4a (TUBA4A), is mapped to human chromosome 2q35. The gene codes for a member of the α-tubulin family containing 448 amino acids. TUBA4A belongs to the subfamily of tubulin, which is the major building block of microtubules. α-subunit of tubulin has molecular weight of 50,000.

Specificity

The antibody recognizes an epitope located on the α3 isoform of Chlamydomonas axonemal α-tubulin, within four residues of Lys-40 when this amino acid is acetylated. A sequence very similar to the one detected by the antibody in Chlamydomonas is found in the majority of α-tubulins, but the corresponding region is markedly divergent in some α-tubulin isoforms from chicken, Drosophila and yeast. The antibody has been used to detect acetylated α-tubulins in several organisms. Occasionally, the epitope recognized by the antibody may be absent or masked as it is in the kangaroo rat epithelial-like cell line PtK2.

Immunogen

acetylated α-tubulin from the outer arm of sea urchin sperm axonemes.

Application

Monoclonal Anti-Tubulin, Acetylated antibody produced in mouse has been used in following studies:
• immunofluorescence.
• western blotting.
• immunocytochemistry.
• immunohistochemistry.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

Acetylated tubulin is associated with axoneme assembly in a variety of organisms. Acetylated tubulin might play a crucial role in the differentiation of microtubule structure and function. Post translational modification of α-tubulin i.e. the reversible addition of an acetyl group to Lys40, by specific acetylase, results in acetylated tubulin.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles

Articles

Antibodies to Tubulins, including Centrosome-Associated ε-Tubulin

Microtubules of the eukaryotic cytoskeleton are composed of a heterodimer of α- and β-tubulin. In addition to α-and β-tubulin, several other tubulins have been identified, bringing the number of dist...
Keywords: Enzyme-linked immunosorbent assay, Gene expression, Western blot

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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