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T7039 Sigma-Aldrich

Transforming Growth Factor-β1 human

TGF-β1, recombinant, expressed in CHO cells, powder, suitable for cell culture

Synonym: TGF-β1, hTGF-β1

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Properties

Related Categories Cancer Research, Cell Biology, Cell Culture, Cell Signaling and Neuroscience, Core Bioreagents,
biological source   human
recombinant   expressed in CHO cells
assay   ≥98% (SDS-PAGE)
form   powder
potency   ≤0.05 ng/mL ED50
quality   endotoxin tested
mol wt   protein 25 kDa
packaging   pkg of 2 μg
  pkg of 50 μg
storage condition   avoid repeated freeze/thaw cycles
impurities   <1 EU/μg
suitability   suitable for cell culture
UniProt accession no.   P01137
storage temp.   −20°C
Gene Information   human ... TGFB1(7040)

Description

General description

Tgfb1 (transforming growth factor-β1 human) is a 25kDa peptide encoded by the gene mapped to human chromosome 19q13.2. TGF-β1 polypeptide is produced as a precursor with hydrophobic signal sequence, pro-region and mature peptide. Cleavage of the precursor at tetrabasic cleavage site results in monomers of carboxy-terminal 112 amino acids. The biologically active form of Tgfb1 exists as a disulphide-linked homodimer. In mammals, three isoforms of TGF-β has been identified : TGF-β1, TGF-β2 and TGF-β3.

Application

Transforming Growth Factor-β1 human has been used in
• reporter gene assay.
• in vitro transfection.
• to study the effect of transforming growth factor-β1 (TGF-β1) on gene array analysis of renal cells.
• to study the association of TGF-β1 with shear stress-dependent upregulation of tissue inhibitor of metalloproteinase-1 in microvascular endothelial cells.
• to study the interaction between TGF-β1 activation and myofibroblast formation.

Biochem/physiol Actions

TGF-β1 is a multifunctional peptide capable of influencing cell proliferation, tissue growth, differentiation, and other functions in a wide range of cell types. TGF-β1 plays a fundamental role in differentiation by involvement in adipogenesis, myogenesis, chondrogenesis, osteogenesis, epithelial cell differentiation and immune cell function. All cells possess a specific TGF-β1 receptor. Both transformed and non-neoplastic tissues release transforming growth factors.The multi-modal nature of TGF-β1 is seen in its ability to stimulate or inhibit cellular proliferation. TGF-β1 is associated with the developmental and metastasis of colorectal cancer.

Transforming growth factor-β1 (TGF-β1) is produced by many cell types, but is reported to be most concentrated in mammalian platelets, where it is present at approximately four times the level of TGF-β2.

Physical form

Lyophilized from a 0.2 μm filtered aqueous solution.

Analysis Note

The biological activity of TGF-β1 is measured in culture by inhibition of mouse IL-4-dependent proliferation of mouse HT-2 cells.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

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Certificate of Origin

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Cell Culture Guide
Protocols & Articles

Articles

Cellular Derived Blood Proteins and Enzymes

Cell damage is induced by reactive oxygen species (ROS). ROS are either free radicals, reactive anions containing oxygen atoms, or molecules containing oxygen atoms that can either produce free radic...
BioFiles 2006, 1.5, 4.
Keywords: Aerobic, Alzheimer Disease, Bacterial conjugations, Biofiles, Cancer, Metabolism, Oxidations, Parkinson Disease, Reductions, Respiration

Growth Factors in Stem Cell Biology

Growth factors are naturally occurring regulatory molecules, which bind to receptors on the cell surface. They stimulate cell and tissue function through influencing cell differentiation by changing ...
Keywords: Adhesion, Angiogenesis, Cell biology, Cell proliferation, Cellular processes, Diseases, Growth factors, Hormones, Stem cell biology, Therapeutic uses, Transcription, Transfection

Naive Pluripotent Stem Cell Culture in an Inhibitor Based 2i/LIF Containing Serum-Free and Feeder-Free Stem Cell Media

Naive pluripotent stem cells are located within the epiblast of mature blastocysts. These primitive “ground-state” cells may be cultured in vitro using specialized media and small molecule inhibitors...
Gabi Tremml, Ming Li and Vi Chu1*,
1MilliporeSigma, Bioscience Division, Temecula, CA, USA
 
Keywords: Cell culture, Gene expression, Immunocytochemistry, Microscopy, Polymerase chain reaction, Polymerase chain reaction - quantitative, Transcription

Transforming Growth Factor (TGF) Superfamily

The transforming growth factor-β (TGF-β) superfamily of cytokines include the structurally related subfamily of TGF-βs, the subfamily of bone morphogenic proteins (BMP), decapentaplegic (dpp) and Vg1...
Jennifer Fries
BioFiles 2009, 4.5, 21.
Keywords: Cancer, Gene expression, Growth factors, Ligands, Phosphorylations, Transduction, Type

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Cell Culture Troubleshooting Guide

Cell culture has become one of the most fundamental techniques for modeling biological systems, and is of increasing importance in the biotechnology and pharmaceutical sectors as well as an essential...
Keywords: Antibiotics, Apoptosis, Cell attachment, Cell culture, Cryopreservation, Filtration, Growth factors, Pharmaceutical, Phase transitions

Peer-Reviewed Papers
15

References

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