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T7451 Sigma-Aldrich

Monoclonal Anti-Acetylated Tubulin antibody produced in mouse

clone 6-11B-1, purified from hybridoma cell culture

Synonym: Acetylated Tubulin Antibody - Monoclonal Anti-Acetylated Tubulin antibody produced in mouse, Acetylated Tubulin Antibody

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies to Cell and Organelle Proteins, Antibodies to Cytoskeleton,
species reactivity   monkey, protista, mouse, pig, human, bovine, invertebrates, rat, hamster, plant, frog, chicken
application(s)   dot blot: suitable
  electron microscopy: suitable
  immunohistochemistry: suitable
  radioimmunoassay: suitable
  western blot: 0.03-0.06 μg/mL using total rat brain extract
clone   6-11B-1, monoclonal
concentration   ~1 mg/mL
antibody form   purified immunoglobulin
form   buffered aqueous solution
isotype   IgG2b
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... TUBA4A(7277)
mouse ... Tuba1a(22142)
rat ... Tuba1a(64158)
biological source   mouse
antibody product type   primary antibodies
conjugate   unconjugated
Featured Industry   Research Pathology
UniProt accession no.   P68366

Description

Immunogen

acetylated tubulin from the outer arm of Strongylocentrotus purpuratus (sea urchin).

General description

α-tubulin, also called as tubulin α 4a (TUBA4A), is mapped to human chromosome 2q35. The gene codes for a member of the α -tubulin family containing 448 amino acids. TUBA4A belongs to the subfamily of tubulin, which is the major building block of microtubules. α-subunit of tubulin has molecular weight of 50,000.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Specificity

The antibody has been used to detect acetylated α-tubulins from many organisms including protista, plants, invertebrates, and vertebrates. The antibody recognizes an epitope located on the α3 isoform of Chlamydomonas axonemal α-tubulin, within four residues of Lys40 when this amino acid is acetylated. A sequence very similar to the one detected by the antibody in Chlamydomonas is found in the majority of α-tubulins, but the corre­sponding region is markedly divergent in some α-tubulin isoforms from chicken, Drosophila, and yeast. Occasionally, the epitope recognized by the antibody may be absent or masked, as it is in the rat kangaroo epithelial-like cell line PtK2.

Application

Monoclonal Anti-Acetylated Tubulin antibody produced in mouse has been used in western blot and immunohistochemistry.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

Acetylated tubulin is associated with axoneme assembly in a variety of organisms. Acetylated tubulin might play a crucial role in the differentiation of microtubule structure and function. Post translational modification of α-tubulin i.e. the reversible addition of an acetyl group to Lys40, by specific acetylase, results in acetylated tubulin.

Safety & Documentation

Documents

Certificate of Analysis

Certificate of Origin

Safety Information

Personal Protective Equipment  Eyeshields, Gloves, half-mask respirator (US), multi-purpose combination respirator cartridge (US)
RIDADR  NONH for all modes of transport
WGK Germany  3
Protocols & Articles

Articles

Antibody Basics

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Protocols

Western Blot Protocol | Immunoblotting Protocol

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Peer-Reviewed Papers
15

References

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