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T7527 Sigma-Aldrich

Tris-Borate-EDTA buffer

BioReagent, for molecular biology, 5x concentrate, DNase and RNase, none detected, powder blend, suitable for electrophoresis

Synonym: TBE buffer

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Properties

Related Categories Biochemicals and Reagents, Biological Buffers, Buffer Convenience Packaging, Buffers A to Z, Molecular Biology,
Quality Level   200
grade   for molecular biology
product line   BioReagent
form   powder blend
impurities   DNase and RNase, none detected
pH   8.1-8.5 (5 ×)
solubility   water: 85.1 g/L, clear, colorless
suitability   suitable for electrophoresis
Featured Industry   Diagnostic Assay Manufacturing
SMILES string   OB(O)O.NC(CO)(CO)CO.OC(=O)CN(CCN(CC(O)=O)CC(O)=O)CC(O)=O
InChI   1S/C10H16N2O8.C4H11NO3.BH3O3/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;5-4(1-6,2-7)3-8;2-1(3)4/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);6-8H,1-3,5H2;2-4H
InChI key   OSBLTNPMIGYQGY-UHFFFAOYSA-N

Description

Packaging

1, 4 L in poly bottle

Application

Ready for use in gel electrophoresis after dilution to working concentrations.

TBE running buffer is the most commonly used buffer for DNA and RNA polyacrylamide gel electrophoresis. TBE is used with non-denaturing or denaturing (7 M urea) gels. It is also routinely used for DNA automated sequencing gel. TBE can also be used for agarose gels but is not recommended for preparative gels for recovery of nucleic acids.
Dilution of the TBE stock concentrates to a 1× TBE running buffer results in a buffer containing 89 mM Tris-borate and 2 mM EDTA, pH 8.3. The 5× or 10× stocks may also be added to an acrylamide/bis-acrylamide stock solution for making the PAGE gel. Applied voltages of less than 5 V/cm (distance between the electrodes of the unit) are recommended for maximum resolution.

Packaging

The 1-liter size is packaged in a plastic bottle large enough to contain the 5× concentrate.

Preparation Note

Prepared with Biotechnology Performance Certified Trizma base (Product Code T6066) and Molecular Biology Reagents boric acid (Product Code B6768) and EDTA disodium salt (Product Code E5134).

Reconstitution

Produces a 5× concentrate (0.445 M Tris-borate, 10 mM EDTA, pH 8.3) after dissolving with the indicated amount of water.

Safety & Documentation

Safety Information

Symbol 
GHS08  GHS08
Signal word 
Danger
Hazard statements 
Precautionary statements 
Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 1
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles

Articles

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Monika Baeumle
BioFiles 2009, 4.1, 4.
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TAE and TBE Running Buffers Recipe & Video

Tris acetate EDTA (TAE) and tris borate EDTA (TBE) are the two most common running buffers used in nucleic acid electrophoresis. As buffers, they have a fairly constant pH and are able to conduct ele...
Keywords: AGE, Buffers, Electrophoresis, Gel electrophoresis, Molecular biology, Peptide synthesis, Polymerase chain reaction, Precipitation, Pulsed field gel electrophoresis, Sequencing

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Peer-Reviewed Papers
15

References

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